LAIR-1 recombinant protein

LAIR-1 (Extracellular domain), 22-125aa, Human, E Coli

Cat. Num. AAA203056

• Gene Names: LAIR1; CD305; LAIR-1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: LAIR-1; LAIR-1 (Extracellular domain); 22-125aa; Human; E Coli; Leukocyte-associated immunoglobulin-like receptor 1 isoform a; Lair1; mLAIR1; CD305 antigen; Leukocyte-associated immunoglobulin-like receptor 1; Leukocyte-associated immunoglobulin-like receptor 1 isoform a 5133400O11Rik; CD305; D7Bwg0421e; LAIR 1; LAIR1 protein; Leukocyte associated Ig like receptor 1; Leukocyte associated immunoglobulin like receptor 1; ; LAIR-1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl (pH 7.5) 50mM NaCl, 1mM EDTA
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MQEEDLPRPS ISAEPGTVIP LGSHVTFVCR GPVGVQTFRL ERESRSTYND TEDVSQASPS ESEARFRIDS VSEGNAGPYR CIYYKPPKWS EQSDYLELLV KETSG
• Sequence Length: 287

• Applicable Applications for LAIR-1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LAIR-1 recombinant protein

LAIR-1 is a glycoprotein (~32kDa) expressed on the surface of the majority of human peripheral blood mononuclear leukocytes including T cells, B cells, NK cells, macrophages and dendritic cells. LAIR-1 functions as an inhibitory receptor in NK cells, T cells and B cells. Lair-1 consists of a leader sequence, extracellular domain, transmembrane domain, cytoplasmic region. The extracellular domain of LAIR-1 (22-125aa) was overexpressed in E Coli, and purified by using conventional chromatography techniques.

Product Categories/Family for LAIR-1 recombinant protein

NK Cell Receptors

References

Sathish JG., et al. (2001) J Immunol. 166 (3):1763-70.; Meyaard L., et al. (1999) J. Immunol. 162, 5800-4.;

NCBI and Uniprot Product Information

• NCBI GI #: 612407859
• NCBI GeneID: 3903
• NCBI Accession #: NP_002278
• NCBI GenBank Nucleotide #: NM_002287.5
• UniProt Accession #: Q6GTX8
• Molecular Weight: 11.7 kDa (105 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform a
• NCBI Official Synonym Full Names: leukocyte associated immunoglobulin like receptor 1
• NCBI Official Symbol: LAIR1
• NCBI Official Synonym Symbols: CD305; LAIR-1
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• UniProt Gene Name: LAIR1
• UniProt Synonym Gene Names: CD305; LAIR-1; hLAIR1

NCBI Description

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.