Western Blot (WB)
(Western Blot analysis of NME5 expression in transfected 293T cell line by NME5 polyclonal antibody. Lane 1: NME5 transfected lysate (24.2kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human NME5 Polyclonal Antibody | anti-NME5 antibody
NME5 (Nucleoside Diphosphate Kinase Homolog 5, NDK-H 5, NDP Kinase Homolog 5, Inhibitor of p53-induced Apoptosis-beta, IPIA-beta, Testis-specific nm23 Homolog, nm23-H5) (Biotin)
Gene Names
NME5; NM23H5; RSPH23; NM23-H5
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NME5; Polyclonal Antibody; NME5 (Nucleoside Diphosphate Kinase Homolog 5; NDK-H 5; NDP Kinase Homolog 5; Inhibitor of p53-induced Apoptosis-beta; IPIA-beta; Testis-specific nm23 Homolog; nm23-H5) (Biotin); anti-NME5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NME5.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-NME5 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human NME5, aa1-212 (NP_003542.1).
Immunogen Sequence
MEISMPPPQIYVEKTLAIIKPDIVDKEEEIQDIILRSGFTIVQRRKLRLSPEQCSNFYVEKYGKMFFPNLTAYMSSGPLVAMILARHKAISYWLELLGPNNSLVAKETHPDSLRAIYGTDDLRNALHGSNDFAAAEREIRFMFPEVIVEPIPIGQAAKDYLNLHIMPTLLEGLTELCKQKPADPLIWLADWLLKNNPNKPKLCHHPIVEEPY
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of NME5 expression in transfected 293T cell line by NME5 polyclonal antibody. Lane 1: NME5 transfected lysate (24.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NME5 expression in transfected 293T cell line by NME5 polyclonal antibody. Lane 1: NME5 transfected lysate (24.2kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-NME5 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-NME5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,236 Da
NCBI Official Full Name
nucleoside diphosphate kinase homolog 5
NCBI Official Synonym Full Names
non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase)
NCBI Official Symbol
NME5
NCBI Official Synonym Symbols
NM23H5; RSPH23; NM23-H5
NCBI Protein Information
nucleoside diphosphate kinase homolog 5; NDK-H 5; IPIA-beta; OTTHUMP00000159495; NDP kinase homolog 5; radial spoke 23 homolog; testis-specific nm23 homolog; inhibitor of p53-induced apoptosis-beta
UniProt Protein Name
Nucleoside diphosphate kinase homolog 5
Protein Family
UniProt Gene Name
NME5
UniProt Synonym Gene Names
NDK-H 5; NDP kinase homolog 5; IPIA-beta
UniProt Entry Name
NDK5_HUMAN
Similar Products
Product Notes
The NME5 nme5 (Catalog #AAA6387163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NME5 (Nucleoside Diphosphate Kinase Homolog 5, NDK-H 5, NDP Kinase Homolog 5, Inhibitor of p53-induced Apoptosis-beta, IPIA-beta, Testis-specific nm23 Homolog, nm23-H5) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NME5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NME5 nme5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NME5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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