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SDS-Page

LAGE3 recombinant protein

LAGE3, 1-143aa, Human, His tag, E Coli

Gene Names
LAGE3; CVG5; ESO3; Pcc1; ITBA2; GAMOS2; DXS9879E; DXS9951E
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
LAGE3; 1-143aa; Human; His tag; E Coli; L antigen family member 3; CVG5; DXS9879E; DXS9951E; ESO3; ITBA2; LAGE3 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMRDADAD AGGGADGGDG RGGHSCRGGV DTAAAPAGGA PPAHAPGPGR DAASAARGSR MRPHIFTLSV PFPTPLEAEI AHGSLAPDAE PHQRVVGKDL TVSGRILVVR WKAEDCRLLR ISVINFLDQL SLVVRTMQRF GPPVSR
Sequence Length
143
Applicable Applications for LAGE3 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for LAGE3 recombinant protein
L antigen family member 3, also known as LAGE3, belongs to the CTAG family. Members of the LAGE/ESO gene family are clustered together on human chromosome Xq28 and have similar exon-intron structures. unlike the other family members, which are normally expressed only in testis and activated in a wide range of human tumors, LAGE3 is ubiquitously expressed in somatic tissues. This protein is also highly conserved in mouse and rat, suggesting that the encoded protein is functionally important. Recombinant human LAGE3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for LAGE3 recombinant protein
References
Alpen B., et al. (2002) Gene. 297:141-149.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17.2kDa (166aa) confirmed by MALDI-TOF
NCBI Official Full Name
EKC/KEOPS complex subunit LAGE3
NCBI Official Synonym Full Names
L antigen family member 3
NCBI Official Symbol
LAGE3
NCBI Official Synonym Symbols
CVG5; ESO3; Pcc1; ITBA2; GAMOS2; DXS9879E; DXS9951E
NCBI Protein Information
EKC/KEOPS complex subunit LAGE3
UniProt Protein Name
EKC/KEOPS complex subunit LAGE3
Protein Family
UniProt Gene Name
LAGE3
UniProt Synonym Gene Names
DXS9879E; ESO3; ITBA2

NCBI Description

This gene belongs to the ESO/LAGE gene family, members of which are clustered together on chromosome Xq28, and have similar exon-intron structures. Unlike the other family members which are normally expressed only in testis and activated in a wide range of human tumors, this gene is ubiquitously expressed in somatic tissues. The latter, combined with the finding that it is highly conserved in mouse and rat, suggests that the encoded protein is functionally important. An intronless pseudogene with high sequence similarity to this gene is located on chromosome 9. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. LAGE3 functions as a dimerization module for the complex ().

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Product Notes

The LAGE3 lage3 (Catalog #AAA204815) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's LAGE3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the LAGE3 lage3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMRDADAD AGGGADGGDG RGGHSCRGGV DTAAAPAGGA PPAHAPGPGR DAASAARGSR MRPHIFTLSV PFPTPLEAEI AHGSLAPDAE PHQRVVGKDL TVSGRILVVR WKAEDCRLLR ISVINFLDQL SLVVRTMQRF GPPVSR. It is sometimes possible for the material contained within the vial of "LAGE3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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