Rabbit anti-Human IKK alpha Polyclonal Antibody | anti-CHUK antibody

IKK alpha Antibody

Cat. Num. AAA151149

• Gene Names: CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: IKK alpha Antibody is affinity chromatography purified via peptide column.
• Synonyms: IKK alpha; Polyclonal Antibody; IKK alpha Antibody; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase alpha; conserved helix-loop-helix ubiquitous kinase; anti-CHUK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This polyclonal antibody has no cross response to IKKb or IKKy.
• Purity/Purification: IKK alpha Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 2273

• Applicable Applications for anti-CHUK antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: IKK alpha antibody can be used for detection of IKK alpha by Western blot 0.5 mug/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IKK alpha antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK alpha.
• Buffer: IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IKK alpha antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IKK alpha in HeLa cell lysate with IKK alpha antibody at 1μg/mL in (A) the absence or (B) the presence of blocking peptide.)

Immunocytochemistry (IHC)

(Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 10 μg/mL. )

Related Product Information for anti-CHUK antibody

IKK alpha Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-alpha. IKKα is expressed in a variety of human tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 2327068
• NCBI GeneID: 1147
• UniProt Accession #: O15111; O14666; Q13132; Q5W0I4; Q92467
• Molecular Weight: 87 kDa
• NCBI Official Full Name: Homo sapiens IkB kinase alpha subunit (IKK alpha) mRNA, complete cds
• NCBI Official Synonym Full Names: conserved helix-loop-helix ubiquitous kinase
• NCBI Official Symbol: CHUK
• NCBI Official Synonym Symbols: IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: CHUK
• UniProt Synonym Gene Names: IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
• UniProt Entry Name: IKKA_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.

Protein type: Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Other group; IKK family

Chromosomal Location of Human Ortholog: 10q24-q25

Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol

Molecular Function: protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; ATP binding; protein kinase activity

Biological Process: skeletal muscle contraction; lactation; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to toxin; response to lipopolysaccharide; osteoclast differentiation; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; Rho protein signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; morphogenesis of an epithelial sheet; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; response to drug; epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; MyD88-independent toll-like receptor signaling pathway; response to virus; mammary gland epithelial cell proliferation; response to amino acid stimulus; odontogenesis of dentine-containing teeth; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to hydroperoxide; toll-like receptor signaling pathway; striated muscle cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation

Disease: Cocoon Syndrome