CD171 / N-CAML1 / L1CAM recombinant protein

Recombinant Human CD171/L1CAM Protein (His Tag)

Cat. Num. AAA2546490

• Gene Names: L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Purity: > 85 % as determined by reducing SDS-PAGE.
• Synonyms: CD171 / N-CAML1 / L1CAM; Recombinant Human CD171/L1CAM Protein (His Tag); CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAML1; S10; SPG1; CD171 / N-CAML1 / L1CAM recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 85 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween 80.
• Sequence: Met 1-Glu 1120

• Application Notes: The recombinant human CD171 consists of 1112 amino acids after removal of the signal peptide and predicts a molecular mass of 125 kDa. As a result of glycosylation, the apparent molecular mass of rhCD171 is approximately 160-200 kDa band in SDS-PAGE under reducing conditions.
• Species: Human
• Tag: C-His
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconsitution: It is recommended that sterile water (400uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for CD171 / N-CAML1 / L1CAM recombinant protein

Background: L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and in a variety of cancer cells, including some common type s of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan and intergrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs) and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.

Description: A DNA sequence encoding the extracellular domain of human CD171 (NP_000416.1) (Met 1-Glu 1120) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4557707
• NCBI GeneID: 3897
• NCBI Accession #: NP_000416.1
• NCBI GenBank Nucleotide #: NM_000425.4
• Molecular Weight: Mol Mass: 125 kDa; AP Mol Mass: 160-200 kDa
• NCBI Official Full Name: neural cell adhesion molecule L1 isoform 1
• NCBI Official Synonym Full Names: L1 cell adhesion molecule
• NCBI Official Symbol: L1CAM
• NCBI Official Synonym Symbols: S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• NCBI Protein Information: neural cell adhesion molecule L1
• UniProt Protein Name: Neural cell adhesion molecule L1
• UniProt Gene Name: L1CAM
• UniProt Synonym Gene Names: CAML1; MIC5; N-CAM-L1; NCAM-L1

NCBI Description

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Uniprot Description

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cell surface; focal adhesion; plasma membrane

Biological Process: axon guidance; chemotaxis; leukocyte migration; nervous system development; positive regulation of axon extension

Disease: Corpus Callosum, Partial Agenesis Of, X-linked; Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Masa Syndrome