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L1-Cell Adhesion Molecule (L1CAM) Recombinant Protein | L1CAM recombinant protein
Recombinant L1-Cell Adhesion Molecule (L1CAM)
Gene Names
L1cam; L1; CD171; NCAM-L1; N-CAM-L1
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
L1-Cell Adhesion Molecule (L1CAM); Recombinant L1-Cell Adhesion Molecule (L1CAM); L1CAM recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SGLRPYSSY HVEVQAFNGR GLGPASEWTF STPEGVPGHP EALHLECQSD TSLLLHWQPP LSHNGVLTGY LLSYHPVEGE SKEQLFFNLS DPELRTHNLT NLNPDLQYRF QLQATTQQGG PGEAIVREGG TMALFGKPDF GNISATAGEN YSVVSWVPRK GQCNFRFHIL FKALPEGKVS PDHQPQPQYV SYNQSSYTQW NLQPDTKYEI HLIKEKVLLH HLDVKTNGTG PVRVSTTGSF ASEGWFIAFV SA
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SGLRPYSSY HVEVQAFNGR GLGPASEWTF STPEGVPGHP EALHLECQSD TSLLLHWQPP LSHNGVLTGY LLSYHPVEGE SKEQLFFNLS DPELRTHNLT NLNPDLQYRF QLQATTQQGG PGEAIVREGG TMALFGKPDF GNISATAGEN YSVVSWVPRK GQCNFRFHIL FKALPEGKVS PDHQPQPQYV SYNQSSYTQW NLQPDTKYEI HLIKEKVLLH HLDVKTNGTG PVRVSTTGSF ASEGWFIAFV SA
Sequence Length
1260
Applicable Applications for L1CAM recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ser882~Ala1132 (Accession # P11627) with two N-terminal Tags, His-tag and GST-tag
Subcellular Location
Cell membrane; Single-pass type I membrane protein
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.1
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
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SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
59.9kDa
NCBI Official Full Name
Neural cell adhesion molecule L1
NCBI Official Synonym Full Names
L1 cell adhesion molecule
NCBI Official Symbol
L1cam
NCBI Official Synonym Symbols
L1; CD171; NCAM-L1; N-CAM-L1
NCBI Protein Information
neural cell adhesion molecule L1
UniProt Protein Name
Neural cell adhesion molecule L1
Protein Family
UniProt Gene Name
L1cam
UniProt Synonym Gene Names
Caml1; N-CAM-L1; NCAM-L1
UniProt Entry Name
L1CAM_MOUSE
Uniprot Description
NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.
Protein type: Membrane protein, integral; Cell adhesion
Cellular Component: presynaptic membrane; focal adhesion; cell surface; membrane; integral to membrane; plasma membrane; terminal button; lipid raft; external side of plasma membrane
Molecular Function: integrin binding; identical protein binding; protein binding; protein self-association; sialic acid binding; PDZ domain binding
Biological Process: axon guidance; nervous system development; multicellular organismal development; positive regulation of calcium-mediated signaling; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; cell-cell adhesion mediated by integrin; positive regulation of cell-cell adhesion; homophilic cell adhesion; cell differentiation; cell adhesion; neurite development
Research Articles on L1CAM
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Product Notes
The L1CAM l1cam (Catalog #AAA2009103) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's L1-Cell Adhesion Molecule (L1CAM) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the L1CAM l1cam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -SGLRPYSSY HVEVQAFNGR GLGPASEWTF STPEGVPGHP EALHLECQSD TSLLLHWQPP LSHNGVLTGY LLSYHPVEGE SKEQLFFNLS DPELRTHNLT NLNPDLQYRF QLQATTQQGG PGEAIVREGG TMALFGKPDF GNISATAGEN YSVVSWVPRK GQCNFRFHIL FKALPEGKVS PDHQPQPQYV SYNQSSYTQW NLQPDTKYEI HLIKEKVLLH HLDVKTNGTG PVRVSTTGSF ASEGWFIAFV SA. It is sometimes possible for the material contained within the vial of "L1-Cell Adhesion Molecule (L1CAM), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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