Importin subunit beta-1 Recombinant Protein | IMB1 recombinant protein

Recombinant mouse Importin subunit beta-1

Cat. Num. AAA9420396

• Gene Names: Kpnb1; IPOB; Impnb; AA409963
• Reactivity: Mouse
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Importin subunit beta-1; Recombinant mouse Importin subunit beta-1; Karyopherin subunit beta-1Nuclear factor p97Pore targeting complex 97 kDa subunit; PTAC97SCG; IMB1 recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-876aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for IMB1 recombinant protein

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.

NCBI and Uniprot Product Information

• NCBI GI #: 88014720
• NCBI GeneID: 16211
• NCBI Accession #: NP_032405.3
• NCBI GenBank Nucleotide #: NM_008379.3
• UniProt Accession #: P70168; Q62117; Q6GTI5
• Molecular Weight: 101.2kD
• NCBI Official Full Name: importin subunit beta-1
• NCBI Official Synonym Full Names: karyopherin (importin) beta 1
• NCBI Official Symbol: Kpnb1
• NCBI Official Synonym Symbols: IPOB; Impnb; AA409963
• NCBI Protein Information: importin subunit beta-1
• UniProt Protein Name: Importin subunit beta-1
• Protein Family: Importin
• UniProt Gene Name: Kpnb1
• UniProt Synonym Gene Names: Impnb; PTAC97

Uniprot Description

KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family.

Protein type: Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 11|11 D

Cellular Component: cytoplasm; cytosol; membrane; nuclear envelope; nuclear membrane; nuclear pore; nucleoplasm; protein complex

Molecular Function: enzyme binding; Hsp90 protein binding; kinesin binding; nuclear localization sequence binding; protein binding; protein complex binding; protein domain specific binding; protein transporter activity; RNA binding

Biological Process: astral microtubule organization and biogenesis; establishment of mitotic spindle localization; establishment of protein localization; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; NLS-bearing substrate import into nucleus; protein import into nucleus; protein import into nucleus, translocation; Ran protein signal transduction; ribosomal protein import into nucleus