Rabbit KPNB1 Polyclonal Antibody | anti-KPNB1 antibody

KPNB1 Polyclonal Antibody

Cat. Num. AAA2519474

• Gene Names: Kpnb1; IPOB; Impnb; AA409963
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: KPNB1; Polyclonal Antibody; KPNB1 Polyclonal Antibody; IMB1; IPO1; IPOB; Impnb; NTF97; anti-KPNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 2.6mg/mL (varies by lot)
• Sequence Length: 34

• Applicable Applications for anti-KPNB1 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human KPNB1
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using KPNB1 Polyclonal Antibody at dilution 1:40)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using KPNB1 Polyclonal Antibody at dilution 1:40)

Related Product Information for anti-KPNB1 antibody

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore.

NCBI and Uniprot Product Information

• NCBI GI #: 13278618
• NCBI GeneID: 16211
• Molecular Weight: 97,184 Da
• NCBI Official Full Name: Kpnb1 protein
• NCBI Official Synonym Full Names: karyopherin (importin) beta 1
• NCBI Official Symbol: Kpnb1
• NCBI Official Synonym Symbols: IPOB; Impnb; AA409963
• NCBI Protein Information: importin subunit beta-1; PTAC97; SCG; importin beta; karyopherin subunit beta-1; nuclear factor p97; pore targeting complex 97 kDa subunit
• UniProt Protein Name: Importin subunit beta-1
• Protein Family: Importin
• UniProt Gene Name: Kpnb1
• UniProt Synonym Gene Names: Impnb; PTAC97
• UniProt Entry Name: IMB1_MOUSE

Uniprot Description

KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family.

Protein type: Nuclear import; Karyopherin

Cellular Component: nucleoplasm; nuclear membrane; protein complex; membrane; cell; cytoplasm; nuclear envelope; intracellular; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; Ran GTPase binding; protein transporter activity

Biological Process: intracellular protein transport; ribosomal protein import into nucleus; protein transport; transport; protein import into nucleus; nucleocytoplasmic transport