Importin subunit beta-1 Recombinant Protein | Kpnb1 recombinant protein

Recombinant Mouse Importin subunit beta-1

Cat. Num. AAA955236

• Gene Names: Kpnb1; IPOB; Impnb; AA409963
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Importin subunit beta-1; Recombinant Mouse Importin subunit beta-1; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; SCG; Kpnb1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-876. Full Length
• Sequence: MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACAEIPVSQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLSTCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGSILEGPEPNQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNILPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDFDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKELRKLKNQA

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for Kpnb1 recombinant protein

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.

References

Localization of the importin-beta gene to mouse chromosome 11D and rat chromosome 10q32.1.Matsuda Y., Hamatani K., Itoh M., Takahashi E., Araki R., Abe M.Genomics 36:213-215(1996) The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile.Imamoto N., Shimamoto T., Kose S., Takao T., Tachibana T., Matsubae M., Sekimoto T., Shimonishi Y., Yoneda Y.FEBS Lett. 368:415-419(1995) Lineage-specific biology revealed by a finished genome assembly of the mouse.Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Multiple pathways contribute to nuclear import of core histones.Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.EMBO Rep. 2:690-696(2001) The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1.Forwood J.K., Harley V., Jans D.A.J. Biol. Chem. 276:46575-46582(2001) Comprehensive identification of phosphorylation sites in postsynaptic density preparations.Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.Mol. Cell. Proteomics 5:914-922(2006) Novel importin-alpha family member Kpna7 is required for normal fertility and fecundity in the mouse.Hu J., Wang F., Yuan Y., Zhu X., Wang Y., Zhang Y., Kou Z., Wang S., Gao S.J. Biol. Chem. 285:33113-33122(2010) The adoption of a twisted structure of importin-beta is essential for the protein-protein interaction required for nuclear transport.Lee S.J., Imamoto N., Sakai H., Nakagawa A., Kose S., Koike M., Yamamoto M., Kumasaka T., Yoneda Y., Tsukihara T.J. Mol. Biol. 302:251-264(2000) The structure of importin-beta bound to SREBP-2 nuclear import of a transcription factor.Lee S.J., Sekimoto T., Yamashita E., Nagoshi E., Nakagawa A., Imamoto N., Yoshimura M., Sakai H., Chong K.T., Tsukihara T., Yoneda Y.Science 302:1571-1575(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 88014720
• NCBI GeneID: 16211
• NCBI Accession #: NP_032405.3
• NCBI GenBank Nucleotide #: NM_008379.3
• UniProt Accession #: P70168; Q62117; Q6GTI5
• Molecular Weight: 101.2 kDa
• NCBI Official Full Name: importin subunit beta-1
• NCBI Official Synonym Full Names: karyopherin (importin) beta 1
• NCBI Official Symbol: Kpnb1
• NCBI Official Synonym Symbols: IPOB; Impnb; AA409963
• NCBI Protein Information: importin subunit beta-1
• UniProt Protein Name: Importin subunit beta-1
• Protein Family: Importin
• UniProt Gene Name: Kpnb1
• UniProt Synonym Gene Names: Impnb; PTAC97
• UniProt Entry Name: IMB1_MOUSE

Uniprot Description

KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family.

Protein type: Karyopherin; Nuclear import

Cellular Component: cytoplasm; membrane; nuclear envelope; nuclear membrane; nuclear pore; nucleoplasm; nucleus; protein complex

Molecular Function: enzyme binding; Hsp90 protein binding; kinesin binding; nuclear localization sequence binding; protein binding; protein complex binding; protein domain specific binding; protein transporter activity; Ran GTPase binding

Biological Process: astral microtubule organization and biogenesis; establishment of mitotic spindle localization; establishment of protein localization; intracellular protein transport; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; NLS-bearing substrate import into nucleus; protein import into nucleus; protein import into nucleus, docking; protein import into nucleus, translocation; protein transport; Ran protein signal transduction; ribosomal protein import into nucleus; transport