Importin subunit beta-1 (Kpnb1) Recombinant Protein | Kpnb1 recombinant protein

Recombinant Rat Importin subunit beta-1 (Kpnb1) , partial

• Gene Names: Kpnb1; Impnb
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Importin subunit beta-1 (Kpnb1); Recombinant Rat Importin subunit beta-1 (Kpnb1); partial; Kpnb1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 875

• Species: Rat
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Kpnb1 recombinant protein

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. This protein is a member of the importin beta family.

NCBI and Uniprot Product Information

• NCBI GI #: 8393610
• NCBI GeneID: 24917
• NCBI Accession #: NP_058759.1
• NCBI GenBank Nucleotide #: NM_017063.1
• UniProt Accession #: P52296
• Molecular Weight: 97,124 Da
• NCBI Official Full Name: importin subunit beta-1
• NCBI Official Synonym Full Names: karyopherin subunit beta 1
• NCBI Official Symbol: Kpnb1
• NCBI Official Synonym Symbols: Impnb
• NCBI Protein Information: importin subunit beta-1
• UniProt Protein Name: Importin subunit beta-1
• Protein Family: Importin
• UniProt Gene Name: Kpnb1
• UniProt Synonym Gene Names: PTAC97

NCBI Description

subunit of complex necessary for nuclear import [RGD, Feb 2006]

Uniprot Description

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Imports SNAI1 and PRKCI into the nucleus ().