Typical Testing Data/Standard Curve (for reference only)
Human Low affinity immunoglobulin gamma Fc region receptor II a (FCGR2A) ELISA Kit | FCGR2A elisa kit
Human Low affinity immunoglobulin gamma Fc region receptor II a (FCGR2A) ELISA Kit
Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Reactivity
Human
Synonyms
Low affinity immunoglobulin gamma Fc region receptor II a (FCGR2A); Human Low affinity immunoglobulin gamma Fc region receptor II a (FCGR2A) ELISA Kit; FCGR2A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of FCGR2A. No significant cross-reactivity or interference between FCGR2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FCGR2A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FCGR2A elisa kit
Intended Uses: This FCGR2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FCGR2A. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: FCGR2A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FCGR2A antibody and an FCGR2A-HRP conjugate. The assay sample and buffer are incubated together with FCGR2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FCGR2A concentration since FCGR2A from samples and FCGR2A-HRP conjugate compete for the anti-FCGR2A antibody binding site. Since the number of sites is limited, as more sites are occupied by FCGR2A from the sample, fewer sites are left to bind FCGR2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FCGR2A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FCGR2A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FCGR2A antibody and an FCGR2A-HRP conjugate. The assay sample and buffer are incubated together with FCGR2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FCGR2A concentration since FCGR2A from samples and FCGR2A-HRP conjugate compete for the anti-FCGR2A antibody binding site. Since the number of sites is limited, as more sites are occupied by FCGR2A from the sample, fewer sites are left to bind FCGR2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FCGR2A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FCGR2A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,930 Da
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-a isoform 2
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIa, receptor (CD32)
NCBI Official Symbol
FCGR2A
NCBI Official Synonym Symbols
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-a; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; Immunoglobulin G Fc receptor II
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-a
Protein Family
UniProt Gene Name
FCGR2A
UniProt Synonym Gene Names
CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a
UniProt Entry Name
FCG2A_HUMAN
NCBI Description
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
Uniprot Description
FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.
Protein type: Receptor, misc.; Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: integral to membrane; plasma membrane
Molecular Function: IgG binding
Biological Process: innate immune response
Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To
Research Articles on FCGR2A
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Product Notes
The Human FCGR2A fcgr2a (Catalog #AAA7236380) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7236380 ELISA Kit recognizes Human FCGR2A. It is sometimes possible for the material contained within the vial of "Low affinity immunoglobulin gamma Fc region receptor II a (FCGR2A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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