NKG2D Recombinant Protein | KLRK1 recombinant protein

NKG2D Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123258

• Gene Names: Klrk1; Nkg2d; NKG2-D; D6H12S2489E
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NKG2D; NKG2D Protein; Mouse; Recombinant (hFc Tag); Mouse NKG2D/CD314 Protein (Fc Tag); D6H12S2489E Protein; NKG2-D Protein; Nkg2d Protein; killer cell lectin-like receptor subfamily K; member 1; KLRK1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 250mM NaCl, pH8.0, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe77-Val219

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse KLRK1 (NP_001076791.1)(Phe77-Val219) was expressed with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KLRK1 recombinant protein

Background: KLRK1 (Killer Cell Lectin Like Receptor K1) is a Protein Coding gene. NKG2D, also known as CD314, is an immune receptor that consists of two disulfide-linked type II transmembrane proteins with short intracellular proteins incapable to transduce signals. To transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.

References

Houchins J, et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J Exp Med. 173: 1017-102.Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428):727-9.Zafirova B, et al. (2011) Regulation of immune cell function and differentiation by the NKG2D receptor. Cell Mol Life Sci. 68(21):3519-29.

NCBI and Uniprot Product Information

• NCBI GI #: 134023658
• NCBI GeneID: 27007
• NCBI Accession #: NP_001076791.1
• NCBI GenBank Nucleotide #: NM_001083322.1
• UniProt Accession #: O54709
• Molecular Weight: 26,710 Da
• NCBI Official Full Name: NKG2-D type II integral membrane protein isoform b
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily K, member 1
• NCBI Official Symbol: Klrk1
• NCBI Official Synonym Symbols: Nkg2d; NKG2-D; D6H12S2489E
• NCBI Protein Information: NKG2-D type II integral membrane protein; NK cell receptor D; natural killer cell group 2D; NKG2-D-activating NK receptor
• UniProt Protein Name: NKG2-D type II integral membrane protein
• Protein Family: NKG2D ligand
• UniProt Gene Name: Klrk1
• UniProt Synonym Gene Names: Nkg2d
• UniProt Entry Name: NKG2D_MOUSE