Mouse Kallikrein-1 ELISA Kit | Klk1 elisa kit

Mouse Kallikrein-1 ELISA Kit

Cat. Num. AAA764267

• Gene Names: KLK1; hK1; KLKR; Klk6
• Reactivity: Mouse
• Synonyms: Kallikrein-1; Mouse Kallikrein-1 ELISA Kit; Klk1/Kallikrein-1/Kidney/pancreas/salivary gland kallikrein/Tissue kallikrein; Klk1 elisa kit

• Reactivity: Mouse
• Sequence Length: 262

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Klk1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Klk1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Klk1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Klk1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Klk1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504875
• NCBI GeneID: 3816
• NCBI Accession #: NP_002248.1
• NCBI GenBank Nucleotide #: NM_002257.3
• Molecular Weight: 23,835 Da
• NCBI Official Full Name: kallikrein-1 preproprotein
• NCBI Official Synonym Full Names: kallikrein 1
• NCBI Official Symbol: KLK1
• NCBI Official Synonym Symbols: hK1; KLKR; Klk6
• NCBI Protein Information: kallikrein-1
• UniProt Protein Name: Kallikrein-1
• Protein Family: Kallikrein
• UniProt Gene Name: KLK1
• UniProt Entry Name: KLK1_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.35; Protease

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleus

Molecular Function: serine-type endopeptidase activity

Biological Process: proteolysis

Disease: Kallikrein, Decreased Urinary Activity Of