Typical Testing Data/Standard Curve (for reference only)
Human KLK1 ELISA Kit | KLK1 elisa kit
Human KLK1 PicoKine ELISA Kit
Gene Names
KLK1; hK1; KLKR; Klk6
Reactivity
Human
Synonyms
KLK1; Human KLK1 PicoKine ELISA Kit; Kallikrein-1; kallikrein 1; Glandular kallikrein 1; hK1; Kallikrein 1 renal/pancreas/salivary; Kallikrein serine protease 1; Kidney/pancreas/salivary gland kallikrein; KLK1_HUMAN; Klk6; KLKR; Tissue kallikrein; KLK1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human KLK1
Sequence Length
262
Samples
Cell culture supernates, cell lysates, serum and plasma(heparin, edta)
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for KLK1 elisa kit
Principle of the Assay: The Picokine™ Human KLK1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK1 with a 96-well strip plate that is pre-coated with antibody specific for KLK1. The detection antibody is a biotinylated antibody specific for KLK1. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK1 with immunogen: Expression system for standard: NSO; Immunogen sequence: I25-S262. The kit is analytically validated with ready to use reagents. To measure Human KLK1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human KLK1 in the sample.
Background/Introduction: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lysbradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Background/Introduction: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lysbradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,835 Da
NCBI Official Full Name
Kallikrein-1
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
KLK1
NCBI Official Synonym Symbols
hK1; KLKR; Klk6
NCBI Protein Information
kallikrein-1; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kallikrein 1, renal/pancreas/salivary; kidney/pancreas/salivary gland kallikrein
UniProt Protein Name
Kallikrein-1
Protein Family
UniProt Gene Name
KLK1
UniProt Entry Name
KLK1_HUMAN
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]
Uniprot Description
KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.21.35
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleus
Molecular Function: serine-type endopeptidase activity
Biological Process: proteolysis
Disease: Kallikrein, Decreased Urinary Activity Of
Research Articles on KLK1
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Product Notes
The Human KLK1 klk1 (Catalog #AAA175837) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175837 ELISA Kit recognizes Human KLK1. It is sometimes possible for the material contained within the vial of "KLK1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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