Porcine Kallikrein 1 ELISA Kit | KLK1 elisa kit

Porcine Kallikrein 1 (KLK1) ELISA Kit

Cat. Num. AAA2604531

• Gene Names: KLK1; hK1; KLKR; Klk6
• Reactivity: Porcine
• Synonyms: Kallikrein 1; Porcine Kallikrein 1 (KLK1) ELISA Kit; KLK1 elisa kit

• Reactivity: Porcine
• Sequence Length: 262

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK1 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine KLK1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504875
• NCBI GeneID: 3816
• NCBI Accession #: NP_002248.1
• NCBI GenBank Nucleotide #: NM_002257.3
• Molecular Weight: 23,835 Da
• NCBI Official Full Name: kallikrein-1 preproprotein
• NCBI Official Synonym Full Names: kallikrein 1
• NCBI Official Symbol: KLK1
• NCBI Official Synonym Symbols: hK1; KLKR; Klk6
• NCBI Protein Information: kallikrein-1
• UniProt Protein Name: Kallikrein-1
• Protein Family: Kallikrein 1-related peptidase
• UniProt Gene Name: KLK1
• UniProt Entry Name: KLK1_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.35; Protease

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleus

Molecular Function: serine-type endopeptidase activity

Biological Process: proteolysis

Disease: Kallikrein, Decreased Urinary Activity Of