Human Kelch-like protein 17 ELISA Kit | KLHL17 elisa kit

Human Kelch-like protein 17 ELISA Kit

Cat. Num. AAA763065

• Reactivity: Human
• Synonyms: Kelch-like protein 17; Human Kelch-like protein 17 ELISA Kit; KLHL17 (Kelch-like protein 17)/Actinfilin; KLHL17 elisa kit

• Reactivity: Human
• Sequence Length: 642

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for KLHL17 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human KLHL17 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KLHL17 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLHL17 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human KLHL17 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38194229
• NCBI GeneID: 339451
• NCBI Accession #: NP_938073.1
• NCBI GenBank Nucleotide #: NM_198317.2
• Molecular Weight: 69,874 Da
• NCBI Official Full Name: kelch-like protein 17
• NCBI Official Synonym Full Names: kelch like family member 17
• NCBI Official Symbol: KLHL17
• NCBI Protein Information: kelch-like protein 17
• UniProt Protein Name: Kelch-like protein 17
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL17
• UniProt Entry Name: KLH17_HUMAN

NCBI Description

The protein encoded by this gene is expressed in neurons of most regions of the brain. It contains an N-terminal BTB domain, which mediates dimerization of the protein, and a C-terminal Kelch domain, which mediates binding to F-actin. This protein may play a key role in the regulation of actin-based neuronal function. [provided by RefSeq, Aug 2010]

Uniprot Description

Actinfilin: Substrate-recognition component of some cullin-RING- based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. The BCR(KLHL17) mediates the ubiquitination and subsequenct degradation of GLUR6. May play a role in the actin-based neuronal function.

Protein type: Actin-binding; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: actin cytoskeleton; cell junction; cell soma; dendrite cytoplasm; extracellular space; postsynaptic density; postsynaptic membrane

Molecular Function: actin filament binding; POZ domain binding; protein complex scaffold; ubiquitin-protein ligase activity

Biological Process: actin cytoskeleton organization and biogenesis; brain development; protein ubiquitination