KIRREL / Kin Of IRRE-Like Protein 1 Recombinant Protein | KIRREL recombinant protein

Human KIRREL / Kin Of IRRE-Like Protein 1 Recombinant Protein

• Gene Names: KIRREL1; NEPH1; KIRREL
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: KIRREL / Kin Of IRRE-Like Protein 1; Human KIRREL / Kin Of IRRE-Like Protein 1 Recombinant Protein; Kin of IRRE-like protein 1; Kin of irregular chiasm-like protein 1; Nephrin-like protein 1; KIRREL1; NEPH1; KIRREL recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 757

• Applicable Applications for KIRREL recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2.
• Entry Function: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 80478236
• NCBI GeneID: 55243
• UniProt Accession #: Q96J84
• NCBI Official Full Name: KIRREL protein
• NCBI Official Synonym Full Names: kirre like nephrin family adhesion molecule 1
• NCBI Official Symbol: KIRREL1
• NCBI Official Synonym Symbols: NEPH1; KIRREL
• NCBI Protein Information: kin of IRRE-like protein 1
• UniProt Protein Name: Kin of IRRE-like protein 1
• Protein Family: Kin of IRRE-like protein
• UniProt Gene Name: KIRREL
• UniProt Synonym Gene Names: KIRREL1; NEPH1
• UniProt Entry Name: KIRR1_HUMAN

NCBI Description

NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1

By similarity.

Subunit structure: Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2

By similarity.

Subcellular location: Cell membrane; Single-pass type I membrane protein

Potential. Note: Predominantly located at podocyte slit diaphragm.

Tissue specificity: Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli. Ref.1

Post-translational modification: Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding

By similarity.N-glycosylated

By similarity.

Sequence similarities: Belongs to the immunoglobulin superfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution: The sequence AAP59845.1 differs from that shown. Reason: Frameshift at position 19. The sequence BAA91850.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14192.1 differs from that shown. Reason: Erroneous initiation.