Human Kin of IRRE-like protein 1 ELISA Kit | KIRREL elisa kit

Human Kin of IRRE-like protein 1 ELISA Kit

Cat. Num. AAA760120

• Gene Names: KIRREL; NEPH1
• Reactivity: Human
• Synonyms: Kin of IRRE-like protein 1; Human Kin of IRRE-like protein 1 ELISA Kit; KIRREL/KIRREL1/NEPH1/kin of IRRE like (Drosophila)/nephrin-like protein 1; KIRREL elisa kit

• Reactivity: Human
• Sequence Length: 757

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for KIRREL elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human KIRREL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KIRREL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KIRREL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human KIRREL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 166295173
• NCBI GeneID: 55243
• NCBI Accession #: NP_060710.3
• NCBI GenBank Nucleotide #: NM_018240.6
• Molecular Weight: 72,360 Da
• NCBI Official Full Name: kin of IRRE-like protein 1 isoform 1
• NCBI Official Synonym Full Names: kin of IRRE like (Drosophila)
• NCBI Official Symbol: KIRREL
• NCBI Official Synonym Symbols: NEPH1
• NCBI Protein Information: kin of IRRE-like protein 1
• UniProt Protein Name: Kin of IRRE-like protein 1
• Protein Family: Kin of IRRE-like protein
• UniProt Gene Name: KIRREL
• UniProt Synonym Gene Names: KIRREL1; NEPH1
• UniProt Entry Name: KIRR1_HUMAN

NCBI Description

NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008]

Uniprot Description

KIRREL: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. Belongs to the immunoglobulin superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell projection membrane; dendritic shaft; integral to membrane; intercellular junction; lipid raft; perinuclear region of cytoplasm; plasma membrane

Molecular Function: myosin binding; protein binding

Biological Process: cell-cell adhesion; excretion; negative regulation of protein amino acid phosphorylation; positive regulation of actin filament polymerization