Western Blot (WB)
((0.1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TSGA2 Polyclonal Antibody | anti-RSPH1 antibody
Goat anti-TSGA2 Antibody
Gene Names
RSPH1; CT79; TSA2; RSP44; TSGA2; RSPH10A
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TSGA2; Polyclonal Antibody; Goat anti-TSGA2 Antibody; RSPH1; radial spoke head 1 homolog (Chlamydomonas); MGC126568; MGC141927; RSP44; RSPH10A; TSA2; h-meichroacidin; male meiotic metaphase chromosome-associated acidic protein; meichroacidin; testes specific A2 homolog; testes specific gene A2 (mou; TSGA2 antibody; RSPH1 antibody; radial spoke head 1 homolog (Chlamydomonas) antibody; MGC126568 antibody; MGC141927 antibody; RSP44 antibody; RSPH10A antibody; TSA2 antibody; h-meichroacidin antibody; male meiotic metaphase chromosome-associated acidic protein antibody; meichroacidin antibody; testes specific A2 homolog antibody; testes specific gene A2 (mouse) homolog antibody; testis specific A2 homolog antibody; testis-specific gene A2 antibody; anti-RSPH1 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HLNHRYQGKFLNKNP
Sequence Length
309
Applicable Applications for anti-RSPH1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 37kDa band observed in Mouse Testis lysates (calculated MW of 35.1kDa according to Human NP_543136.1 and 34.2kDa according to Mouse NP_079566.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 37kDa band observed in Mouse Testis lysates (calculated MW of 35.1kDa according to Human NP_543136.1 and 34.2kDa according to Mouse NP_079566.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-HLNHRYQGKFLNKNP, from the internal region of the protein sequence according to NP_543136.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,847 Da
NCBI Official Full Name
radial spoke head 1 homolog isoform 1
NCBI Official Synonym Full Names
radial spoke head 1 homolog
NCBI Official Symbol
RSPH1
NCBI Official Synonym Symbols
CT79; TSA2; RSP44; TSGA2; RSPH10A
NCBI Protein Information
radial spoke head 1 homolog
UniProt Protein Name
Radial spoke head 1 homolog
Protein Family
UniProt Gene Name
RSPH1
UniProt Synonym Gene Names
TSA2; TSGA2; CT79
UniProt Entry Name
RSPH1_HUMAN
NCBI Description
This gene encodes a male meiotic metaphase chromosome-associated acidic protein. This gene is expressed in tissues with motile cilia or flagella, including the trachea, lungs, airway brushings, and testes. Mutations in this gene result in primary ciliary dyskinesia-24. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
TSGA2: May play an important role in male meiosis.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: condensed nuclear chromosome; cytosol; nucleus; outer dense fiber
Molecular Function: protein binding
Biological Process: axoneme biogenesis; meiotic cell cycle; spermatid development
Disease: Ciliary Dyskinesia, Primary, 24
Research Articles on RSPH1
Similar Products
Product Notes
The RSPH1 rsph1 (Catalog #AAA422040) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TSGA2 Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TSGA2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 37kDa band observed in Mouse Testis lysates (calculated MW of 35.1kDa according to Human NP_543136.1 and 34.2kDa according to Mouse NP_079566.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the RSPH1 rsph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HLNHRYQGKF LNKNP. It is sometimes possible for the material contained within the vial of "TSGA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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