Typical Testing Data/Standard Curve (for reference only)
Human Ketohexokinase ELISA Kit | KHK elisa kit
Human Ketohexokinase ELISA Kit
Reactivity
Human
Synonyms
Ketohexokinase; Human Ketohexokinase ELISA Kit; Hepatic fructokinase; KHK; 2.7.1.3; KHK elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural human Ketohexokinase. No significant cross-reactivity or interference was observed.
Sequence Length
298
Samples
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.31 ng/mL -20.0 ng/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for KHK elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Ketohexokinase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ketohexokinase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ketohexokinase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ketohexokinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ketohexokinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ketohexokinase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ketohexokinase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ketohexokinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ketohexokinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,730 Da
NCBI Official Full Name
ketohexokinase isoform a
NCBI Official Synonym Full Names
ketohexokinase
NCBI Official Symbol
KHK
NCBI Protein Information
ketohexokinase
UniProt Protein Name
Ketohexokinase
Protein Family
UniProt Gene Name
KHK
UniProt Entry Name
KHK_HUMAN
NCBI Description
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
Research Articles on KHK
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Product Notes
The Human KHK khk (Catalog #AAA2882613) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882613 ELISA Kit recognizes Human KHK. It is sometimes possible for the material contained within the vial of "Ketohexokinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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