Rabbit anti-Human RDH13 Polyclonal Antibody | anti-RDH13 antibody

RDH13, ID (RDH13, Retinol dehydrogenase 13) (Azide free) (HRP)

Cat. Num. AAA6341140

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RDH13; Polyclonal Antibody; ID (RDH13; Retinol dehydrogenase 13) (Azide free) (HRP); Retinol dehydrogenase 13; anti-RDH13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 260

• Applicable Applications for anti-RDH13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RDH13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-202 amino acids from the Central region of human RDH13.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RDH13 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RDH13 antibody detected the RDH13 protein (arrow).)

Flow Cytometry (FC/FACS)

(RDH13 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RDH13 antibody

This gene encodes a mitochondrial short-chain dehydrogenase/reductase, which catalyzes the reduction and oxidation of retinoids. The encoded enzyme may function in retinoic acid production and may also protect the mitochondria against oxidative stress. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-RDH13 antibody

Antibodies; Enzymes; Dehydrogenase

References

Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009): Belyaeva, O.V., et al. FEBS J. 275(1):138-147(2008). Haeseleer, F., et al. J. Biol. Chem. 277(47):45537-45546(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 19923931
• NCBI Accession #: NP_612421.1
• NCBI GenBank Nucleotide #: NP_612421.1
• UniProt Accession #: Q8NBN7
• NCBI Official Full Name: retinol dehydrogenase 13 isoform 2
• UniProt Protein Name: Retinol dehydrogenase 13
• Protein Family: Retinol dehydrogenase
• UniProt Gene Name: RDH13
• UniProt Synonym Gene Names: PSEC0082; UNQ736/PRO1430
• UniProt Entry Name: RDH13_HUMAN