Western Blot (WB)
Rabbit Neu Polyclonal Antibody
Neu (C18) Antibody
Reactivity
Mouse, rat, human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Synonyms
Neu; Polyclonal Antibody; Neu (C18) Antibody; anti-Neu antibody
Host
Rabbit
Reactivity
Mouse, rat, human
Clonality
Polyclonal
Specificity
Mouse, rat, and human Neu
Form/Format
Each vial contain 200ug/ml of affinity purified rabbit IgG Neu (C18), in 1ml PBS containing 0.1% sodium azide and 0.2% gelatin.
Concentration
0.200 mg/ml (varies by lot)
Applicable Applications for anti-Neu antibody
Western Blot (WB), Immunopreciptation (IP), Immunohistochemistry (IHC) Paraffin
Application Notes
Western blot analysis of Neu expression in SK-BR-3 whole cell lysates.
Western Blotting: 1:200
Western Blotting: 1:200
Origin
Neu (C18) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human Neu (HER2 or ErbB-2).
Immunogen
Synthetic peptide mapping to the carboxy terminus of human Neu (HER2 or ErbB-2).
Competition studies
A blocking peptide is also available for use in competition studies. Each vial contains 100ug of peptide in 0.5ml PBS with 0.1% sodium azide and 100 ug BSA.
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-Neu antibody
The Neu/HER-2 (also known as EGFR-2 or ErbB-2) is a transmembrane tyrosine kinase receptor and a well-defined tumor antigen. Neu/HER-2 is a shared antigen among multiple tumor types. Patients with HER-2/neu protein-overexpressing breast, ovarian, non-small cell lung, colon, and prostate cancers have been shown to have a pre-existent immune response to Neu/HER-2 (1,2). Neu/HER-2 amplification/overexpression confers more aggressive and malignant characteristics on breast cancer cells. Patients with Neu/HER-2-amplified breast cancer have a worse prognosis than those with normal Neu/HER-2 expression (3).
References
1. Bernhard H, Salazar L, Schiffman K, Smorlesi A, Schmidt B, Knutson KL, Disis ML. Vaccination against the HER-2/neu oncogenic protein. Int J Endocr Relat Cancer 2002 Mar;9(1):33-44.2. Lohrisch C, Piccart M. HER2/neu as a predictive factor in breast cancer. Clin Breast Cancer 2001 Jul;2(2):129-35; discussion 136-7.3. Hortobagyi GN. Overview of treatment results with trastuzumab (Herceptin) in metastatic breast cancer. Semin Oncol 2001 Dec;28(6 Suppl 18):43-7
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Product Notes
The Neu (Catalog #AAA440046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neu (C18) Antibody reacts with Mouse, rat, human and may cross-react with other species as described in the data sheet. AAA Biotech's Neu can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunopreciptation (IP), Immunohistochemistry (IHC) Paraffin. Western blot analysis of Neu expression in SK-BR-3 whole cell lysates. Western Blotting: 1:200. Researchers should empirically determine the suitability of the Neu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neu, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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