SDS-PAGE
(The purity of KDM1A was determined to be >95% by densitometry. Approx. MW 100kDa.)
KDM1A recombinant protein
KDM1A recombinant protein
SDS-PAGE
(The purity of KDM1A was determined to be >95% by densitometry. Approx. MW 100kDa.)
SDS-PAGE
(The purity of KDM1A was determined to be >95% by densitometry. Approx. MW 100kDa.)
Scientific Background: KDM1A or lysine (K)-specific demethylase 1A is a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain that is a component of several histone deacetylase complexes and act as a histone demethylase. (1) H3K4 histone demethylase activity of KDM1A is partly responsible for the repressive activity of TAL1 and restricts TAL1 function in hematopoiesis (1). KDM1A plays an essential role for CoREST in demethylation of H3K4 both in vitro and in vivo (2).
2. Lee, M. G. et.al : An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation. Nature 437: 432-435, 2005
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Uniprot Description
Function: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. Ref.5 Ref.8 Ref.12 Ref.15 Ref.23 Ref.24
Cofactor: FAD. Ref.8 Ref.9
Subunit structure: Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B
By similarity. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Interacts with SNAI1 (via SNAG domain). Ref.5 Ref.6 Ref.7 Ref.10 Ref.11 Ref.12 Ref.22 Ref.24
Subcellular location: Nucleus Ref.6 Ref.12.
Tissue specificity: Ubiquitously expressed. Ref.12
Domain: The SWIRM domain may act as an anchor site for a histone tail. Ref.30
Sequence similarities: Belongs to the flavin monoamine oxidase family.Contains 1 SWIRM domain.
Biophysicochemical propertiesKinetic parameters:KM=3.0 µM for H3 monomethyl-K4 Ref.13KM=4.2 µM for H3 dimethyl-K4KM=3.9 µM for H3 monomethyl-K4-monomethyl-K9KM=17.5 µM for monomethyl-K4-acetyl-K9
Sequence caution: The sequence BAA25527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Research Articles on KDM1A
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Product Notes
The KDM1A kdm1a (Catalog #AAA515219) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human KDM1A (172-end) was expressed by baculovirus in Sf9 insect cells!!Gene Acession Number||BC048134. AAA Biotech's KDM1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the KDM1A kdm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM1A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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