Human Potassium channel subfamily K member 4 ELISA Kit | KCNK4 elisa kit

Human Potassium channel subfamily K member 4 (KCNK4) ELISA Kit

Cat. Num. AAA280397

• Gene Names: KCNK4; TRAAK; K2p4.1; TRAAK1
• Reactivity: Human
• Synonyms: Potassium channel subfamily K member 4; Human Potassium channel subfamily K member 4 (KCNK4) ELISA Kit; KCNK4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KCNK4. No significant cross-reactivity or interference between Human KCNK4 and analogues was observed.
• Sequence Length: 393

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for KCNK4 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNK4 in samples. An antibody specific for KCNK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNK4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 952977797
• NCBI GeneID: 50801
• NCBI Accession #: NP_001304019.1
• NCBI GenBank Nucleotide #: NM_001317090.1
• UniProt Accession #: Q9NYG8; Q96T94; B5TJL1
• Molecular Weight: 45,173 Da
• NCBI Official Full Name: potassium channel subfamily K member 4
• NCBI Official Synonym Full Names: potassium two pore domain channel subfamily K member 4
• NCBI Official Symbol: KCNK4
• NCBI Official Synonym Symbols: TRAAK; K2p4.1; TRAAK1
• NCBI Protein Information: potassium channel subfamily K member 4
• UniProt Protein Name: Potassium channel subfamily K member 4
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK4
• UniProt Synonym Gene Names: TRAAK; Two pore K(+) channel KT4.1

NCBI Description

This gene encodes a member of the TWIK-related arachidonic acid-stimulated two pore potassium channel subfamily. The encoded protein homodimerizes and functions as an outwardly rectifying channel. This channel is regulated by polyunsaturated fatty acids, temperature and mechanical deformation of the lipid membrane. This protein is expressed primarily in neural tissues and may be involved in regulating the noxious input threshold in dorsal root ganglia neurons. Alternate splicing results in multiple transcript variants. Naturally occurring read-through transcripts also exist between this gene and the downstream testis expressed 40 (TEX40) gene, as represented in GeneID: 106780802. [provided by RefSeq, Nov 2015]

Uniprot Description

KCNK4: Voltage insensitive, instantaneous, outwardly rectifying potassium channel. Outward rectification is reversed at high external K(+) concentrations. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: potassium channel activity; potassium ion leak channel activity; voltage-gated ion channel activity

Biological Process: detection of mechanical stimulus involved in sensory perception of touch; memory; potassium ion transport; sensory perception of pain; sensory perception of temperature stimulus; stabilization of membrane potential