Mouse Inter alpha-trypsin inhibitor, heavy chain 4 ELISA Kit | Itih4 elisa kit

Mouse Inter alpha-trypsin inhibitor, heavy chain 4 ELISA Kit

Cat. Num. AAA2885293

• Gene Names: Itih4; Itih-4; PK-120; ITI-HC4
• Reactivity: Mouse
• Synonyms: Inter alpha-trypsin inhibitor; heavy chain 4; Mouse Inter alpha-trypsin inhibitor; heavy chain 4 ELISA Kit; ITI heavy chain H4; Itih4; Itih4 elisa kit

• Reactivity: Mouse
• Sequence Length: 941

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Itih4 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226531069
• NCBI GeneID: 16427
• NCBI Accession #: NP_001152771.1
• NCBI GenBank Nucleotide #: NM_001159299.2
• UniProt Accession #: A6X935; O54882; Q505P8; Q8C7G9; Q8C7K5; Q91W60; Q9DBK8
• Molecular Weight: 100,395 Da
• NCBI Official Full Name: inter alpha-trypsin inhibitor, heavy chain 4 isoform 2
• NCBI Official Synonym Full Names: inter alpha-trypsin inhibitor, heavy chain 4
• NCBI Official Symbol: Itih4
• NCBI Official Synonym Symbols: Itih-4; PK-120; ITI-HC4
• NCBI Protein Information: inter alpha-trypsin inhibitor, heavy chain 4
• UniProt Protein Name: Inter alpha-trypsin inhibitor, heavy chain 4
• Protein Family: Inter alpha-trypsin inhibitor
• UniProt Gene Name: Itih4
• UniProt Synonym Gene Names: ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4
• UniProt Entry Name: ITIH4_MOUSE

NCBI Description

This gene encodes a member of the inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors with diverse functions as anti-apoptotic and matrix stabilizing molecules during development. This gene is predominantly expressed in the liver and the encoded protein was found to be a plasma kallikrein-sensitive glycoprotein. This gene is located in a cluster of related inter alpha trypsin inhibitor genes on chromosome 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Uniprot Description

ITIH4: May be involved in acute phase reactions. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: cytoplasm; extracellular region; plasma membrane

Molecular Function: calcium ion binding; hyaluronic acid binding; protease inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; hyaluronan metabolic process; negative regulation of peptidase activity