Human FGFBP1 ELISA Kit | FGFBP1 elisa kit

Human FGFBP1 (Fibroblast Growth Factor Binding Protein 1) ELISA Kit

Cat. Num. AAA2503420

• Gene Names: FGFBP1; FGFBP; HBP17; FGF-BP; FGF-BP1; FGFBP-1
• Reactivity: Human
• Synonyms: FGFBP1; Human FGFBP1 (Fibroblast Growth Factor Binding Protein 1) ELISA Kit; FGFBP1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human FGFBP1. No significant cross-reactivity or interference between Human FGFBP1 and analogues was observed.
• Sequence Length: 234

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGFBP1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FGFBP1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGFBP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FGFBP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGFBP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FGFBP1. You can calculate the concentration of FGFBP1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4826754
• NCBI GeneID: 9982
• NCBI Accession #: NP_005121.1
• NCBI GenBank Nucleotide #: NM_005130.4
• UniProt Accession #: Q14512; A8K5J2
• Molecular Weight: 26,264 Da
• NCBI Official Full Name: fibroblast growth factor-binding protein 1
• NCBI Official Synonym Full Names: fibroblast growth factor binding protein 1
• NCBI Official Symbol: FGFBP1
• NCBI Official Synonym Symbols: FGFBP; HBP17; FGF-BP; FGF-BP1; FGFBP-1
• NCBI Protein Information: fibroblast growth factor-binding protein 1; 17 kDa HBGF-binding protein; 17 kDa heparin-binding growth factor-binding protein; FGF-binding protein 1; heparin-binding growth factor binding protein
• UniProt Protein Name: Fibroblast growth factor-binding protein 1
• Protein Family: Fibroblast growth factor-binding protein
• UniProt Gene Name: FGFBP1
• UniProt Synonym Gene Names: FGFBP; HBP17; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; 17 kDa HBGF-binding protein; HBp17
• UniProt Entry Name: FGFP1_HUMAN

NCBI Description

This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]

Uniprot Description

FGFBP1: Acts as a carrier protein that release fibroblast- binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth. Belongs to the fibroblast growth factor-binding protein family.

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: extracellular space; cell surface; plasma membrane

Molecular Function: heparin binding; protein binding; fibroblast growth factor binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; signal transduction