Human inter-alpha (globulin) inhibitor H2 ELISA Kit | ITIH2 elisa kit

Human Inter-alpha-trypsin inhibitor heavy chain H2, ITIH2 ELISA Kit

Cat. Num. AAA943401

• Gene Names: ITIH2; H2P; SHAP
• Reactivity: Human
• Synonyms: inter-alpha (globulin) inhibitor H2; Human Inter-alpha-trypsin inhibitor heavy chain H2; ITIH2 ELISA Kit; Human Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA kit; RP11-264C14.2; H2P; SHAP; ITI heavy chain H2; inter-alpha (globulin) inhibitor; H2 polypeptide; inter-alpha globulin inhibitor H2 polypeptide; inter-alpha-inhibitor heavy chain 2; inter-alpha-trypsin in; ITIH2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ITIH2. No significant cross-reactivity or interference between human ITIH2 and analogues was observed.
• Sequence Length: 946

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 3.12 ug/ml-200 ug/ml.
• Sensitivity: 0.78 ug/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITIH2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITIH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITIH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITIH2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITIH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 70778918
• NCBI GeneID: 3698
• NCBI Accession #: NP_002207.2
• NCBI GenBank Nucleotide #: NM_002216.2
• UniProt Accession #: P19823; Q14659; Q15484; Q5T986
• Molecular Weight: 106,463 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H2
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 2
• NCBI Official Symbol: ITIH2
• NCBI Official Synonym Symbols: H2P; SHAP
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H2; ITI-HC2; ITI heavy chain H2; inter-alpha (globulin) inhibitor H2; inter-alpha-inhibitor heavy chain 2; serum-derived hyaluronan-associated protein; inter-alpha (globulin) inhibitor, H2 polypeptide; inter-alpha-trypsin inhibitor complex component II
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H2
• Protein Family: Inter-alpha-trypsin inhibitor
• UniProt Gene Name: ITIH2
• UniProt Synonym Gene Names: IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP
• UniProt Entry Name: ITIH2_HUMAN

NCBI Description

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]

Uniprot Description

ITIH2: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: hyaluronan metabolic process