Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) RTU ELISA Kit | ITIH2 rtu elisa kit

Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) ELISA Kit

Cat. Num. AAA4500531

• Gene Names: ITIH2; H2P; SHAP
• Reactivity: Human
• Synonyms: Inter Alpha-Globulin Inhibitor H2 (ITIH2); Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) ELISA Kit; H2P; SHAP; IGHEP2; Serum-derived hyaluronan-associated protein; Inter-alpha-trypsin inhibitor complex component II; ITIH2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 946

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 35pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITIH2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITIH2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ITIH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITIH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ITIH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 70778918
• NCBI GeneID: 3698
• NCBI Accession #: NP_002207.2
• NCBI GenBank Nucleotide #: NM_002216.2
• UniProt Accession #: P19823; Q14659; Q15484; Q5T986
• Molecular Weight: 106,463 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H2
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 2
• NCBI Official Symbol: ITIH2
• NCBI Official Synonym Symbols: H2P; SHAP
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H2
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H2
• Protein Family: Inter-alpha-trypsin inhibitor
• UniProt Gene Name: ITIH2
• UniProt Synonym Gene Names: IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP

NCBI Description

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]

Uniprot Description

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.