Typical Testing Data/Standard Curve
Canine Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit | ITIH2 elisa kit
Canine Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||ITIH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ITIH2 antibody and an ITIH2-HRP conjugate. The assay sample and buffer are incubated together with ITIH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITIH2 concentration since ITIH2 from samples and ITIH2-HRP conjugate compete for the anti-ITIH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITIH2 from the sample, fewer sites are left to bind ITIH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITIH2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]
Uniprot Description
ITIH2: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.
Protein type: Secreted; Inhibitor; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10p15
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity
Biological Process: hyaluronan metabolic process
Research Articles on ITIH2
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Product Notes
The Canine ITIH2 itih2 (Catalog #AAA7238813) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7238813 ELISA Kit recognizes Canine ITIH2. It is sometimes possible for the material contained within the vial of "Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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