Integrin beta-1 Recombinant Protein | ITB1 recombinant protein

Recombinant Rat Integrin beta-1

Cat. Num. AAA9420372

• Reactivity: Rat
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Integrin beta-1; Recombinant Rat Integrin beta-1; Beta oligodendroglia; Beta OLFibronectin receptor subunit betaVLA-4 subunit beta; CD29; ITB1 recombinant protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Extracellular Domain, 21-729aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for ITB1 recombinant protein

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma mbranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assbly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis.

NCBI and Uniprot Product Information

• NCBI GI #: 158303324
• NCBI GeneID: 24511
• NCBI Accession #: NP_058718.2
• NCBI GenBank Nucleotide #: NM_017022.2
• UniProt Accession #: P49134; A2RRT8
• Molecular Weight: 94.4kD
• NCBI Official Full Name: integrin beta-1
• NCBI Official Synonym Full Names: integrin subunit beta 1
• NCBI Official Symbol: Itgb1
• NCBI Protein Information: integrin beta-1
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: Itgb1
• UniProt Synonym Gene Names: Beta OL

NCBI Description

beta subunit of integrin receptor which binds collagen [RGD, Feb 2006]

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: acrosome; adherens junction; basement membrane; cell surface; cytoplasm; cytoplasmic vesicle; dendritic spine; external side of plasma membrane; filopodium; focal adhesion; hemidesmosome; integrin complex; intercellular junction; lipid raft; membrane; neuromuscular junction; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; receptor complex; sarcolemma; synapse

Molecular Function: actin binding; alpha-actinin binding; C-X3-C chemokine binding; cadherin binding; cell adhesion molecule binding; collagen binding; fibronectin binding; glycoprotein binding; integrin binding; kinase binding; laminin binding; peptide binding; protease binding; protein binding; protein complex binding; protein domain specific binding; protein heterodimerization activity; protein kinase binding; receptor binding

Biological Process: axon extension; calcium-independent cell-matrix adhesion; cardiac muscle cell differentiation; cardiac muscle development; cell adhesion; cell adhesion mediated by integrin; cell fate specification; cell migration; cell migration during sprouting angiogenesis; cell-cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; cellular calcium ion homeostasis; dendrite morphogenesis; formation of radial glial scaffolds; G1/S transition of mitotic cell cycle; germ cell migration; heterotypic cell-cell adhesion; in utero embryonic development; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte tethering or rolling; maternal process involved in pregnancy; mesodermal cell differentiation; negative regulation of cell differentiation; negative regulation of cell projection organization and biogenesis; negative regulation of cell proliferation; negative regulation of neuron differentiation; negative regulation of Rho protein signal transduction; neurite development; positive regulation of apoptosis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endocytosis; positive regulation of GTPase activity; positive regulation of MAPKKK cascade; positive regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; protein transport within lipid bilayer; receptor internalization; regulation of cell cycle; regulation of G-protein coupled receptor protein signaling pathway; response to activity; response to drug; response to nutrient levels; sarcomere organization; stress fiber formation; tissue homeostasis; transforming growth factor beta receptor signaling pathway; visual learning