Integrin Beta 1 (ITGb1) Recombinant Protein | ITGb1 recombinant protein

Recombinant Integrin Beta 1 (ITGb1)

Cat. Num. AAA2009824

• Gene Names: Itgb1; CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Integrin Beta 1 (ITGb1); Recombinant Integrin Beta 1 (ITGb1); ITGb1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VEVVLQF ICKCNCQSHG IPASPKCHEG NGTFECGACR CNEGRVGRHC ECSTDEVNSE DMDAYCRKEN SSEICSNNGE CVCGQCVCRK RDNTNEIYSG KFCECDNFNC DRSNGLICGG NGVCRCRVCE CYPNYTGSAC DCSLDTGPCL ASNGQICNGR GICECGACKC TDPKFQGPTC ETCQTCLGVC AEHKECVQCR AFNKGEKKDT CAQECSHFNL TKVESREKLP QPVQVDPVTH CKEKDIDDCW FYFTYSVNGN NEAI
• Sequence Length: 798

• Applicable Applications for ITGb1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val454~Ile714 (Accession # P09055) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 45504394
• NCBI GeneID: 16412
• NCBI Accession #: NP_034708.1
• NCBI GenBank Nucleotide #: NM_010578.2
• UniProt Accession #: P09055; Q3TIW5; Q3TWH6; Q60993; Q61126; Q8BTU0; Q8BVU1; F6R105
• Molecular Weight: 32.5kDa
• NCBI Official Full Name: integrin beta-1
• NCBI Official Synonym Full Names: integrin beta 1 (fibronectin receptor beta)
• NCBI Official Symbol: Itgb1
• NCBI Official Synonym Symbols: CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907
• NCBI Protein Information: integrin beta-1; VLA-4 subunit beta; fibronectin receptor subunit beta
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: Itgb1
• UniProt Entry Name: ITB1_MOUSE

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Membrane protein, integral; Cell surface; Motility/polarity/chemotaxis; Receptor, misc.; Cell adhesion

Cellular Component: cell surface; integral to membrane; intercellular junction; lipid raft; membrane; hemidesmosome; perinuclear region of cytoplasm; cytoplasm; basement membrane; synapse; cytoplasmic vesicle; integrin complex; neuromuscular junction; endosome; receptor complex; external side of plasma membrane; filopodium; focal adhesion; dendritic spine; acrosome; cell projection; adherens junction; plasma membrane; cell junction; sarcolemma

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; receptor activity; actin binding; alpha-actinin binding; peptide binding; protein kinase binding; collagen binding; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; protein complex binding; cell adhesion molecule binding; glycoprotein binding; kinase binding; receptor binding

Biological Process: axon extension; regulation of cell cycle; positive regulation of apoptosis; multicellular organismal development; cell-matrix adhesion; positive regulation of endocytosis; cell fate specification; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; germ cell migration; positive regulation of cell proliferation; tissue homeostasis; visual learning; cell adhesion; neurite development; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; cardiac muscle development; cell migration; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of neuron differentiation; negative regulation of cell differentiation; cell migration during sprouting angiogenesis; negative regulation of Rho protein signal transduction; stress fiber formation; calcium-independent cell-matrix adhesion; positive regulation of neuron differentiation; leukocyte tethering or rolling; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of cell migration; G1/S transition of mitotic cell cycle