Integrin alpha 5 beta 1 Recombinant Protein | ITGA5&ITGB1 recombinant protein

Integrin alpha 5 beta 1 Protein, Human, Recombinant (DYKDDDDK & His Tag)

Cat. Num. AAA8123606

• Gene Names: ITGA5; FNRA; CD49e; VLA5A
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Integrin alpha 5 beta 1; Integrin alpha 5 beta 1 Protein; Human; Recombinant (DYKDDDDK & His Tag); Human ITGA5 & ITGB1 Heterodimer Protein; CD49e Protein; FNRA Protein; VLA-5 Protein; VLA5A Protein; integrin; alpha 5 (fibronectin receptor; alpha polypeptide); ITGA5&ITGB1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr995&Met1-Asp728

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 42 & Gln 21
• Tag: C-DYKDDDDK & C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Tyr 995) of human ITGA5 (P08648) was fused with a DYKDDDDK tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Product Categories/Family for ITGA5&ITGB1 recombinant protein

AKT Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 56237029
• NCBI GeneID: 3678
• NCBI Accession #: NP_002196.2
• NCBI GenBank Nucleotide #: NM_002205.2
• UniProt Accession #: P08648; P05556
• Molecular Weight: 114,536 Da
• NCBI Official Full Name: integrin alpha-5
• NCBI Official Synonym Full Names: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• NCBI Official Symbol: ITGA5
• NCBI Official Synonym Symbols: FNRA; CD49e; VLA5A
• NCBI Protein Information: integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit
• UniProt Protein Name: Integrin alpha-5
• UniProt Gene Name: ITGA5
• UniProt Synonym Gene Names: FNRA
• UniProt Entry Name: ITA5_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 12q11-q13

Cellular Component: ruffle; focal adhesion; cell surface; cytoplasm; plasma membrane; synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: integrin binding; protein binding; vascular endothelial growth factor receptor 2 binding; metal ion binding; platelet-derived growth factor receptor binding; epidermal growth factor receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; extracellular matrix organization and biogenesis; viral reproduction; cell-substrate adhesion; positive regulation of vascular endothelial growth factor receptor signaling pathway; memory; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion mediated by integrin; cell-substrate junction assembly; angiogenesis; cell adhesion; blood coagulation; leukocyte migration; wound healing, spreading of epidermal cells; positive regulation of cell migration