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Western Blot (WB) (CMAS Antibody (C-term) western blot analysis in U87-MG cell line lysates (35ug/lane).This demonstrates the CMAS antibody detected the CMAS protein (arrow).)

Rabbit CMAS Polyclonal Antibody | anti-CMAS antibody

CMAS Antibody (C-term)

Gene Names
CMAS; CSS
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CMAS; Polyclonal Antibody; CMAS Antibody (C-term); N-acylneuraminate cytidylyltransferase; CMP-N-acetylneuraminic acid synthase; CMP-NeuNAc synthase; anti-CMAS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CMAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-327 amino acids from the C-terminal region of human CMAS.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
300-327
Sequence Length
434
Applicable Applications for anti-CMAS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Cellular Location
Nucleus
Tissue Location
Ubiquitously expressed. Expressed in pancreas, kidney, liver, skeletal muscle, lung, placenta, brain, heart, colon, PBL, small intestine, ovary, testis, prostate, thymus, and spleen.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(CMAS Antibody (C-term) western blot analysis in U87-MG cell line lysates (35ug/lane).This demonstrates the CMAS antibody detected the CMAS protein (arrow).)

Western Blot (WB) (CMAS Antibody (C-term) western blot analysis in U87-MG cell line lysates (35ug/lane).This demonstrates the CMAS antibody detected the CMAS protein (arrow).)
Related Product Information for anti-CMAS antibody
Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).
Product Categories/Family for anti-CMAS antibody
References
Lawrence S.M., et al. Glycoconj. J. 18:205-213(2001).
Bouquin T., et al. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Ota T., et al. Nat. Genet. 36:40-45(2004).
Bechtel S., et al. BMC Genomics 8:399-399(2007).
Bienvenut W.V., et al. Submitted (AUG-2009) to UniProtKB.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48379
NCBI Official Full Name
N-acylneuraminate cytidylyltransferase
NCBI Official Synonym Full Names
cytidine monophosphate N-acetylneuraminic acid synthetase
NCBI Official Symbol
CMAS
NCBI Official Synonym Symbols
CSS
NCBI Protein Information
N-acylneuraminate cytidylyltransferase
UniProt Protein Name
N-acylneuraminate cytidylyltransferase
UniProt Gene Name
CMAS
UniProt Synonym Gene Names
CMP-NeuNAc synthase
UniProt Entry Name
NEUA_HUMAN

NCBI Description

Sialic acids are a family of nine-carbon sugars on cell surface glycoproteins and glycolipids that play a pivotal role in determining the structure and function of many animal tissues. The pattern of cell surface sialylation is highly regulated during embryonic development and N-glycosylation is a common post-translational modification during cellular differentiation. Sialic acids play important roles in cell-cell communications and immune responses. Sialylated glycoprotein and glycolipid formation requires the activation of a sialic acid to a cytidine monophosphate (CMP) diester by the enzyme encoded by this gene: CMP-N-acetylneuraminic acid synthetase. [provided by RefSeq, Jul 2012]

Uniprot Description

CMAS: Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Belongs to the CMP-NeuNAc synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.43; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: N-acylneuraminate cytidylyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; N-acetylneuraminate metabolic process

Similar Products

Product Notes

The CMAS cmas (Catalog #AAA9207740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 300-327. The CMAS Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's CMAS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CMAS cmas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CMAS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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