Testing Data
VEGF-E, Heparin-binding Active Protein | VEGF-E active protein
Orf Virus VEGF-E, Heparin-binding
Reactivity
Orf virus
Purity
> 90% by SDS-PAGE & silver stain
Synonyms
VEGF-E; Heparin-binding; Orf Virus VEGF-E; Recombinant ov-HB-Vascular Endothelial Growth Factor-E; ov-HB-VEGF-E; VEGF-E active protein
Host
Insect Cells
Reactivity
Orf virus
Purity/Purification
> 90% by SDS-PAGE & silver stain
Form/Format
Lyophilized
Sequence
N-Terminal Sequence: UNDER WORK!
Sequence Length
154
Buffer
50 mM acetic acid
Preparation and Storage
Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Reconstituted HB-VEGF-E should be stored in working aliquots at -20 degree C.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for VEGF-E active protein
A DNA sequence encoding the first 116 amino acid residue of Orf virus VEGF-E isolate D1701 (Dehio et al., 1999 EMBO J. 18:363-374; GenBank accession No. AF106020) was fused with a DNA sequence encoding to the C-terminal heparin binding domain of human VEGF165. The chimeric protein was expressed in insect cells using a baculovirus expression system. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994 J. Virol 68:84-92). Different isolates of orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appear to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999 EMBO J. 18:363-374; Wise et al., 1999 Proc. Natl. Acad. Sci USA 96:3071-3076). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E and hb-VEGF-E can not bind to VEGF receptor-1 (Flt-1). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor -2/ KDR. Compared to human VEGF165 this virus form has no heparin-binding domain and seems to be a freely secreted protein comparable to VEGF121. In order to compare this form with human VEGF165, an additional heparin-binding domain was engineered at the C-terminus to allow interaction with proteo-aminoglycans and heparan sulfate. These form is also able to interact with neuropillin -1.
Product Categories/Family for VEGF-E active protein
References
1. Dehio et al., 1999 EMBO J. 18:363-374 2. Lyttle et al., 1994 J. Virol 68:84-92 3. Wise et al., 1999 Proc. Natl. Acad. Sci USA 96:3071-3076
NCBI and Uniprot Product Information
Uniprot Description
Sequence similarities: Belongs to the PDGF/VEGF growth factor family. RuleBase RU003818
Similar Products
Product Notes
The VEGF-E (Catalog #AAA691480) is an Active Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The Orf Virus VEGF-E, Heparin-binding reacts with Orf virus and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: N-Terminal Sequence: UNDER WORK!. It is sometimes possible for the material contained within the vial of "VEGF-E, Heparin-binding, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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