VEGF-C active protein

Rat VEGF-C

Cat. Num. AAA691817

• Reactivity: Rat
• Purity: > 90% by SDS-PAGE & silver stain
• Synonyms: VEGF-C; Rat VEGF-C; Recombinant Rat Vascular Endothelial Growth Factor-C; VRP; Flt4-L; VEGF-related protein; VEGF-C active protein

• Host: Insect Cells
• Reactivity: Rat
• Purity/Purification: > 90% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: SIDNEWRKTQ AND NEWRKTQCMP
• Sequence Length: 127

• N Terminal Sequence: SIDNEWRKTQ AND NEWRKTQCMP
• Buffer: 50 mM acetic acid
• Preparation and Storage: Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Reconstituted VEGF-C should be stored in working aliquots at -20 degree C. Avoid repeated freeze-thaw cycles!

Testing Data

Testing Data #2

Testing Data #3

Related Product Information for VEGF-C active protein

VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 127 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 15-20 kDa protein in SDS-PAGE under reducing conditions.

Product Categories/Family for VEGF-C active protein

Cytokines & Growth Factors

References

1. Joukov et al., EMBO J 15:290, 1996 2. Olofsson et al., Curr Opin Biotech 10:528, 1999 3. Kukk et al., Development 122:3829, 1996

NCBI and Uniprot Product Information

• NCBI GI #: 16758992
• NCBI GeneID: 114111
• NCBI Accession #: NP_446105.1
• NCBI GenBank Nucleotide #: NM_053653.1
• UniProt Accession #: O35757; Q91ZE3
• Molecular Weight: 15-20 kDa
• NCBI Official Full Name: vascular endothelial growth factor C
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: Vegfc
• NCBI Protein Information: vascular endothelial growth factor C; VRP; VEGF-C; flt4-L; flt4 ligand; vascular endothelial growth factor-related protein
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: Vegfc
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_RAT

NCBI Description

platelet-derived growth factor/vascular derived growth factor (PDGF/VEGF); active in angiogenesis and endothelial cell growth [RGD, Feb 2006]

Uniprot Description

Function: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

Subunit structure: Homodimer; non-covalent and antiparallel.

Subcellular location: Secreted.

Tissue specificity: Highly expressed in the lung, ovary, preputial gland and the adrenal gland. Expressed in the post-pubertal mammary glands. Ref.1 Ref.2

Developmental stage: Increases moderately during pregnancy (2.8-fold increase on day 4) and lactation (1.9-fold increase on day 2). Ref.2

Post-translational modification: Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-223 and Ser-224 producing a heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions

By similarity.

Miscellaneous: Indolinones; MAE87, MAE106 and MAZ51 inhibit VEGF-C induced activation of VEGFR-3.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.