BACE2 active protein

BACE2 (soluble) (human), (recombinant) (His-tag)

Cat. Num. AAA566693

• Gene Names: BACE2; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• Purity: >=80% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: BACE2; BACE2 (soluble) (human); (recombinant) (His-tag); BACE2 active protein

• Host: Insect cells
• Purity/Purification: >=80% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 50mM TRIS-HCl, pH7.5, containing 100mM sodium chloride and 20% glycerol
• Sequence: aaAla21-Pro466

• Source Note: Recombinant soluble BACE2, cloned from human cDNA, secreted as zymogen from insect cells, purified using a C-terminal His-tag, and cleaved to the mature active form. Produced in a baculovirus expression system
• Specific Activity: >=20U/ug enzyme. One unit will hydrolyze one pmole Mca-KPLGL-Dpa-AR substrate (10uM) per minute at 37 degree C, in 50mM NaOAc, pH4.5, 1M sodium chloride
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; BACE2 is stable after 5 freeze-thaws at 0.3-0.4ug//ul; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity; Avoid freeze/thaw cycles; Shipping: Shipped on Dry Ice

Related Product Information for BACE2 active protein

BACE2 is an aspartic protease found in both transmembrane and soluble forms. It is related to, and possesses an overlapping substrate profile with, BACE1. It is likely involved in Alzheimer's disease, and implicated in other neurological, pancreatic, and muscle pathologies.

Product Categories/Family for BACE2 active protein

Proteostasis; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• NCBI GI #: 6685260
• NCBI GeneID: 25825
• UniProt Accession #: Q9Y5Z0; Q5DIH8; Q8N2D4; Q9H2V8; Q9NZL1; Q9NZL2; Q9UJT6; A8K7P1
• Molecular Weight: 518
• NCBI Official Full Name: Beta-secretase 2
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 2
• NCBI Official Symbol: BACE2
• NCBI Official Synonym Symbols: ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• NCBI Protein Information: beta-secretase 2; memapsin-1; theta-secretase; aspartyl protease 1; 56 kDa aspartic-like protease; Down syndrome region aspartic protease; transmembrane aspartic proteinase Asp1; membrane-associated aspartic protease 1; beta-site amyloid beta A4 precursor
• UniProt Protein Name: Beta-secretase 2
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE2
• UniProt Synonym Gene Names: AEPLC; ALP56; ASP21; ASP1; Asp 1; Beta-site APP cleaving enzyme 2; DRAP
• UniProt Entry Name: BACE2_HUMAN

NCBI Description

This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer's disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BACE2: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C- terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Belongs to the peptidase A1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.45; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to membrane; endosome

Molecular Function: aspartic-type endopeptidase activity

Biological Process: negative regulation of amyloid precursor protein biosynthetic process; membrane protein ectodomain proteolysis; peptide hormone processing; proteolysis