Human beta secretase 2 (BACE2) ELISA Kit | BACE2 elisa kit

Human beta secretase 2 (BACE2) ELISA Kit

Cat. Num. AAA7201281

• Gene Names: BACE2; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• Reactivity: Human
• Synonyms: beta secretase 2 (BACE2); Human beta secretase 2 (BACE2) ELISA Kit; Human b secretase 2 (BACE2) ELISA Kit; BACE2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This BACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BACE2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BACE2 elisa kit

Principle of the assay: BACE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BACE2 antibody and an BACE2-HRP conjugate. The assay sample and buffer are incubated together with BACE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BACE2 concentration since BACE2 from samples and BACE2-HRP conjugate compete for the anti-BACE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BACE2 from the sample, fewer sites are left to bind BACE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BACE2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BACE2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 19923395
• NCBI GeneID: 25825
• NCBI Accession #: NP_036237.2
• NCBI GenBank Nucleotide #: NM_012105.4
• Molecular Weight: 46,476 Da
• NCBI Official Full Name: beta-secretase 2 isoform A preproprotein
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 2
• NCBI Official Symbol: BACE2
• NCBI Official Synonym Symbols: ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1
• NCBI Protein Information: beta-secretase 2; memapsin-1; theta-secretase; aspartyl protease 1; 56 kDa aspartic-like protease; Down syndrome region aspartic protease; transmembrane aspartic proteinase Asp1; membrane-associated aspartic protease 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2
• UniProt Protein Name: Beta-secretase 2
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE2
• UniProt Synonym Gene Names: AEPLC; ALP56; ASP21; CDA13; UNQ418/PRO852; ASP1; Asp 1; Beta-site APP cleaving enzyme 2; DRAP
• UniProt Entry Name: BACE2_HUMAN

NCBI Description

This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer's disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BACE2: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C- terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Belongs to the peptidase A1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.45; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to membrane; endosome

Molecular Function: aspartic-type endopeptidase activity

Biological Process: negative regulation of amyloid precursor protein biosynthetic process; peptide hormone processing; membrane protein ectodomain proteolysis; proteolysis