Western Blot (WB)
(Host: RabbitTarget Name: LST8Sample Type: Fetal Liver lysatesAntibody Dilution: 1.0ug/ml)
Rabbit anti-Human MLST8 Polyclonal Antibody | anti-MLST8 antibody
MLST8 Antibody - N-terminal region
Western Blot (WB)
(Host: RabbitTarget Name: LST8Sample Type: Fetal Liver lysatesAntibody Dilution: 1.0ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: LST8Sample Type: Fetal Liver lysatesAntibody Dilution: 1.0ug/ml)
NCBI and Uniprot Product Information
Uniprot Description
LST8: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum- induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12- rapamycin. Interacts directly with MTOR and RPTOR. Interacts with RHEB. Broadly expressed, with highest levels in skeletal muscle, heart and kidney. Belongs to the WD repeat LST8 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: protein binding
Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of actin filament polymerization; nerve growth factor receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway
Research Articles on MLST8
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Product Notes
The MLST8 mlst8 (Catalog #AAA3220091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLST8 Antibody - N-terminal region reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLST8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the MLST8 mlst8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: TRTVQHQDSQ VNALEVTPDR SMIAAAGYQH IRMYDLNSNN PNPIISYDGV. It is sometimes possible for the material contained within the vial of "MLST8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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