Human Sestrin 2 (SESN2) ELISA Kit | SESN2 elisa kit

Human Sestrin 2 (SESN2) ELISA Kit

Cat. Num. AAA166783

• Gene Names: SESN2; HI95; SES2; SEST2
• Reactivity: Human
• Synonyms: Sestrin 2 (SESN2); Human Sestrin 2 (SESN2) ELISA Kit; SESN2 elisa kit

• Reactivity: Human
• Sequence Length: 480

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05-15ng/ml
• Sensitivity: 0.01ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SESN2 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Sestrin 2 (also known as SESN2) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SESN2 antibody. SESN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SESN2 Antibody is added and binds to SESN2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SESN2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SESN2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 21707433
• NCBI GeneID: 83667
• Molecular Weight: 54,494 Da
• NCBI Official Full Name: Sestrin 2
• NCBI Official Synonym Full Names: sestrin 2
• NCBI Official Symbol: SESN2
• NCBI Official Synonym Symbols: HI95; SES2; SEST2
• NCBI Protein Information: sestrin-2
• UniProt Protein Name: Sestrin-2
• Protein Family: Sestrin
• UniProt Gene Name: SESN2

NCBI Description

This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. [provided by RefSeq, Jul 2008]

Uniprot Description

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling (PubMed:18692468, PubMed:25263562, PubMed:25457612, PubMed:26449471, PubMed:26612684, PubMed:26586190). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:26449471, PubMed:26586190). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468). Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed:26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (PubMed:19113821).