Structure
(Structure of C1036-70.)
Calyculin A, Inhibitor
Calyculin A (Serine/Threonine Phosphatase Inhibitor)
Purity
Highly Purified>= 98% by HPLC and TLC
Synonyms
Calyculin A; Calyculin A (Serine/Threonine Phosphatase Inhibitor); inhibitor
Purity/Purification
Highly Purified
>= 98% by HPLC and TLC
>= 98% by HPLC and TLC
Application Notes
Inhibition of protein serine/threonine phosphatases (PP-1 and PP-2) Potent non-phorbol type tumor promotion.
CAS Number
101932-71-2
Activity
The protein phosphatase inhibitory activity is tested on adipocyte cytosolic protein phosphatase activity, using myelin basic protein phosphorylated by protein kinase C as a substrate. An Okadaic acid-resistant phosphatase is inhibited with ID50 of 200nM, while 1uM exerts full inhibition of total phosphatase activity.
Molecular Formula
C50H81N4O15P
Solubility
Soluble in DMSO and EtOH; insoluble in water. Calyculin A must first be dissolved in an organic solvent such as DMSO, ethanol, acetone or methanol. The recommended solvent for stock solutions to be used for biological experiments is DMSO.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with DMSO and EtOH. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not expose to sunlight or fluorescent lights for extended periods.
Warning! This product is a suspect cancer hazard and tumor promoter. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure.
Warning! This product is a suspect cancer hazard and tumor promoter. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure.
Structure
(Structure of C1036-70.)
Structure
(Structure of C1036-70.)
Related Product Information for Calyculin A, inhibitor
Calyculin A, a polyene phosphate, was initially isolated from the marine sponge Discodermia calyx. It is a very potent, cell-permeable PP-1 and PP-2 inhibitor (IC50: 2nM and 0.5-1nM respectively, and extremely specific for PP-1 and PP2A classes of protein serine/threonine phosphatase among other acidic, alkaline and protein phosphatases. Calyculin A is a polyene phosphate, a potent inhibitor (Ki ~ 0.1nM) with high specificity for the PP-1 and PP-2 classes of protein serine/threonine phosphatase.
Product Categories/Family for Calyculin A, inhibitor
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
1009.18D
NCBI Official Full Name
Chain B, Crystal Structure Of The Complex Between Calyculin A And The Catalytic Subunit Of Protein Phosphatase 1
Similar Products
Product Notes
The Calyculin A (Catalog #AAA655318) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. Inhibition of protein serine/threonine phosphatases (PP-1 and PP-2) Potent non-phorbol type tumor promotion. Researchers should empirically determine the suitability of the Calyculin A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calyculin A, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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