Testing Data
(Lane 1: MW MarkerLane 2: PEGylated protein)
Rat PEG Monoclonal Antibody | anti-PEG antibody
Anti-PEG Antibody (Clone 26A04)
Reactivity
All. It reacts with PEGylated proteins such as PEG-GCSF, PEG-Erythropoietin, and PEG-Interferon, but does not react to unmodified form of the same proteins.
Applications
ELISA, Western Blot
Purity
Ceramic hydroxyapatite (CHT) chromatography from in vitro production
Synonyms
PEG; Monoclonal Antibody; Anti-PEG Antibody (Clone 26A04); Polyethylene Glycol; anti-PEG antibody
Host
Rat
Reactivity
All. It reacts with PEGylated proteins such as PEG-GCSF, PEG-Erythropoietin, and PEG-Interferon, but does not react to unmodified form of the same proteins.
Clonality
Monoclonal
Isotype
IgM
Clone Number
26A04
Purity/Purification
Ceramic hydroxyapatite (CHT) chromatography from in vitro production
Form/Format
1 mg/ml in TBS, pH 9.0.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-PEG antibody
ELISA (EIA), Western Blot (WB), Serves as Positive Controls for Drmug Detection Assays.
Immunogen
PEG-KLH
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
Store at -20 to -80°C; avoid repeat freeze-thaw cycles
Testing Data
(Lane 1: MW MarkerLane 2: PEGylated protein)
Testing Data
(Lane 1: MW MarkerLane 2: PEGylated protein)
Testing Data
(An Indirect ELISA was run against a panel of three PEG conjugates and one unconjugated PEG (20 kDa). The PEG reagents were coated at 5 µg/ml and the antibodies were run at 2 µg/ml. The data shows that both MBS840003 and MBS840032 bind specifically to PEG in this application. Previous work also demonstrated that these antibodies are specific for the linear backbone of PEG and do not bind to the methoxy group.)
Testing Data
(An Indirect ELISA was run against a panel of three PEG conjugates and one unconjugated PEG (20 kDa). The PEG reagents were coated at 5 µg/ml and the antibodies were run at 2 µg/ml. The data shows that both MBS840003 and MBS840032 bind specifically to PEG in this application. Previous work also demonstrated that these antibodies are specific for the linear backbone of PEG and do not bind to the methoxy group.)
Related Product Information for anti-PEG antibody
Polyethylene Glycol (PEG) is a polymer of ethylene oxide available in size variants from 400 Da to 40 kDa. It is nonionic, nontoxic, biocompatible, strong, and hydrophilic and has a large exclusion volume in aqueous solution. PEG has a number of industrial and biomedical applications. The modification of a biopharmaceutical with PEG increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. In particular, therapeutic proteins are conjugated with PEG to slow down their clearance from circulation and improve bioavailability. PEG antibodies can be a vital tool for propelling therapeutics to market by serving as a positive control anti-drug antibody, measuring clearance of a drug, or simply as a QA release confirming PEGylation.
Product Categories/Family for anti-PEG antibody
Similar Products
Product Notes
The PEG (Catalog #AAA840032) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-PEG Antibody (Clone 26A04) reacts with All. It reacts with PEGylated proteins such as PEG-GCSF, PEG-Erythropoietin, and PEG-Interferon, but does not react to unmodified form of the same proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's PEG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Serves as Positive Controls for Drmug Detection Assays. Researchers should empirically determine the suitability of the PEG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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