Testing Data
(A431 cells were treated with different concentrations of Okadaic Acid and C1036-72 for 30 minutes; total cell extracts were then blotted with Phospho-Threonine Antibody (P-Thr-Polyclonal).)
Calyculin A, Inhibitor
Calyculin A (Serine/Threonine Phosphatase Inhibitor)
Synonyms
Calyculin A; Calyculin A (Serine/Threonine Phosphatase Inhibitor); inhibitor
Form/Format
Supplied as a lyophilized clear film. For 10uM stock, reconstitute in 1ml DMSO.
Application Notes
Suitable for use as an inhibitor of protein phosphatases PP1 and PP2A. Other applications not tested
Dilution: Treat cells with 50-100nM calyculin A for 5-30 minutes.
Optimal dilutions to be determined by the researcher
Dilution: Treat cells with 50-100nM calyculin A for 5-30 minutes.
Optimal dilutions to be determined by the researcher
Preparation and Storage
Reconstitute lyophilized clear film. with 1ml DMSO, aliquot, tightly seal and store at -20 degree C in the dark. Reconstituted product is stable for 6 months at -20 degree C.
Testing Data
(A431 cells were treated with different concentrations of Okadaic Acid and C1036-72 for 30 minutes; total cell extracts were then blotted with Phospho-Threonine Antibody (P-Thr-Polyclonal).)
Testing Data
(A431 cells were treated with different concentrations of Okadaic Acid and C1036-72 for 30 minutes; total cell extracts were then blotted with Phospho-Threonine Antibody (P-Thr-Polyclonal).)
Western Blot (WB)
(Western Blot analysis of whole cell lysates of Jurkat cells untreated and treated with 0.1uM C1036-72 for 45 minutes prior to lysis, using Phospho-Threonine Antibody (P-Thr-Polyclonal). Proteins were separated by 2D electrophoresis prior to blotting.)
Western Blot (WB)
(Western Blot analysis of whole cell lysates of Jurkat cells untreated and treated with 0.1uM C1036-72 for 45 minutes prior to lysis, using Phospho-Threonine Antibody (P-Thr-Polyclonal). Proteins were separated by 2D electrophoresis prior to blotting.)
Related Product Information for Calyculin A, inhibitor
Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, calyculin A inhibits both phosphatases (1). Neither calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phosphotyrosine protein phosphatases (2). Calyculin A (Serine/Threonine Phosphatase Inhibitor) is a much more potent phosphatase inhibitor than Okadaic acid (2). As shown in the first Western blot, treating cells with 100nM Calyculin A for 30 minutes can dramatically induce threonine phosphorylation as detected by this antibody. IC50 values for inhibitory activity against PP1 are around 2nM. IC50 values for inhibitory activity against PP2A are around 0.5-1nM.
Product Categories/Family for Calyculin A, inhibitor
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
1009.18
NCBI Official Full Name
Chain B, Crystal Structure Of The Complex Between Calyculin A And The Catalytic Subunit Of Protein Phosphatase 1
Similar Products
Product Notes
The Calyculin A (Catalog #AAA655481) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. Suitable for use as an inhibitor of protein phosphatases PP1 and PP2A. Other applications not tested Dilution: Treat cells with 50-100nM calyculin A for 5-30 minutes. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Calyculin A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calyculin A, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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