Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pappalysin-2 (PAPPA2) Standard Curve.)
Human Pappalysin-2 (PAPPA2) ELISA Kit | PAPPA2 elisa kit
Human Pappalysin-2 (PAPPA2) ELISA Kit
Gene Names
PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2
Reactivity
Human
Synonyms
Pappalysin-2 (PAPPA2); Human Pappalysin-2 (PAPPA2) ELISA Kit; PAPPA2; RP4-774I24.1; PAPP-A2; PAPPE; PLAC3; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; pregnancy-associated plasma protein-E; PAPPA2 elisa kit
Reactivity
Human
Specificity
Human Pappalysin-2 (PAPPA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAPPA2. No significant cross-reactivity or interference between Human PAPPA2 and analogues was observed.
Sequence Length
1791
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pappalysin-2 (PAPPA2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pappalysin-2 (PAPPA2) Standard Curve.)
Related Product Information for PAPPA2 elisa kit
Description: This Human Pappalysin-2 (PAPPA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PAPPA2 in samples. An antibody specific for PAPPA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAPPA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAPPA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAPPA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pappalysins comprise one of the five families of Metzincins (other families include ADAMs/ADAMTSs, MMPs, Astacins, and Serralysins). Pappalysin-1 (PAPP-A) is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy. Reduced maternal serum PAPP-A is a major marker of Down syndrome in the first trimester of pregnancy. PAPP-A cleaves IGFBP-4 and -5 (insulin-like growth factor-binding protein-4 and 5) at a single site, resulting in the release of bioactive IGF. Pappalysin-2 (PAPP-A2; also called PAPP-E) shares 45% amino acid identity with PAPP-A and cleaves IGFBP-2.sialic acid binding Ig-like lectin 6 (Siglec-6), a potential leptin receptor, and pappalysin-2 (PAPP-A2), a protease that cleaves IGF-binding proteins
Background: Pappalysins comprise one of the five families of Metzincins (other families include ADAMs/ADAMTSs, MMPs, Astacins, and Serralysins). Pappalysin-1 (PAPP-A) is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy. Reduced maternal serum PAPP-A is a major marker of Down syndrome in the first trimester of pregnancy. PAPP-A cleaves IGFBP-4 and -5 (insulin-like growth factor-binding protein-4 and 5) at a single site, resulting in the release of bioactive IGF. Pappalysin-2 (PAPP-A2; also called PAPP-E) shares 45% amino acid identity with PAPP-A and cleaves IGFBP-2.sialic acid binding Ig-like lectin 6 (Siglec-6), a potential leptin receptor, and pappalysin-2 (PAPP-A2), a protease that cleaves IGF-binding proteins
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pappalysin-2 isoform 1
NCBI Official Synonym Full Names
pappalysin 2
NCBI Official Symbol
PAPPA2
NCBI Official Synonym Symbols
PAPPE; PLAC3; PAPP-E; PAPP-A2
NCBI Protein Information
pappalysin-2
UniProt Protein Name
Pappalysin-2
Protein Family
UniProt Gene Name
PAPPA2
UniProt Synonym Gene Names
PLAC3; PAPP-A2; PAPP-E
UniProt Entry Name
PAPP2_HUMAN
NCBI Description
This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
Uniprot Description
PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.-; Protease; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1q25.2
Cellular Component: membrane; extracellular region; intracellular
Molecular Function: metallopeptidase activity; zinc ion binding
Biological Process: cellular protein metabolic process; regulation of cell growth; cell differentiation; proteolysis
Research Articles on PAPPA2
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Product Notes
The Human PAPPA2 pappa2 (Catalog #AAA9716022) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716022 ELISA Kit recognizes Human PAPPA2. It is sometimes possible for the material contained within the vial of "Pappalysin-2 (PAPPA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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