Aquaporin-1 (AQP1) Active Protein | AQP1 active protein

Recombinant Human Aquaporin-1 (AQP1) (Active)

Cat. Num. AAA7007848

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Aquaporin-1 (AQP1); Recombinant Human Aquaporin-1 (AQP1) (Active); Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule; AQP1 active protein

• Host: In vitro E Coli expression system
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid or Lyophilized Powder; If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, p
• Sequence Positions: 2-269aa; Full Length of Mature Protein
• Sequence: ASEFKKKLFWRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSLGRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK

• Species: Human
• Tag: N-terminal 6xHis-SUMO-tagged
• Endotoxin: Not test.
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized AQP1 at 2 ug/ml can bind human RGS17, the EC50 of human RGS17 protein is 31.63-34.44 ug/ml.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

SDS-Page

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

Related Product Information for AQP1 active protein

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Product Categories/Family for AQP1 active protein

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 297307116
• UniProt Accession #: P29972
• Molecular Weight: 44.4 kDa
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear membrane; integral to plasma membrane; basolateral plasma membrane; brush border membrane; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; ephrin receptor binding; potassium channel activity; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; nitric oxide transporter activity; water channel activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; cellular response to stress; water transport; carbon dioxide transport; negative regulation of caspase activity; pancreatic juice secretion; sensory perception of pain; positive regulation of saliva secretion; odontogenesis; positive regulation of fibroblast proliferation; hyperosmotic salinity response; nitric oxide transport; bicarbonate transport; multicellular organismal water homeostasis; cellular homeostasis; transmembrane transport; potassium ion transport; lipid digestion; lateral ventricle development; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; renal water homeostasis; cell volume homeostasis; negative regulation of apoptosis

Disease: Blood Group--colton