Mouse Erp57 (Grp58) Monoclonal Antibody | anti-PDIA3 antibody

Erp57 (Grp58) Antibody: PerCP

Cat. Num. AAA803258

• Gene Names: PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• Reactivity: Human, Mouse, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: Erp57 (Grp58); Monoclonal Antibody; Erp57 (Grp58) Antibody: PerCP; ERp60; ERp61; Grp57; Grp58; P58; PDIA3; PI PLC; anti-PDIA3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: Map.ERP57
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 505

• Applicable Applications for anti-PDIA3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: 1:2000 (WB)
• Conjugate: PerCP
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis and Hair Follicles.)

Western Blot (WB)

(Western Blot analysis of Human cell lysates showing detection of Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining.)

Related Product Information for anti-PDIA3 antibody

Background Info: Detects ~57kDa.

Scientific Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Product Categories/Family for anti-PDIA3 antibody

Heat Shock; Organelle Markers

References

1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
1. Lynes, E.M. et al. (2013). Palmitoylation is the Switch that Assigns Calnexin to Quality Control or ER Calcium Signaling. J Cell Sci. Advance Online Article July 10, 2013. doi: 10.1242/jcs.125856

NCBI and Uniprot Product Information

• NCBI GI #: 21361657
• NCBI GeneID: 2923
• NCBI Accession #: NP_005304.3
• NCBI GenBank Nucleotide #: NM_005313.4
• UniProt Accession #: P30101; Q13453; Q14255; Q8IYF8; Q9UMU7; AAB37397
• Molecular Weight: 56,782 Da
• NCBI Official Full Name: protein disulfide-isomerase A3
• NCBI Official Synonym Full Names: protein disulfide isomerase family A, member 3
• NCBI Official Symbol: PDIA3
• NCBI Official Synonym Symbols: P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• NCBI Protein Information: protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
• UniProt Protein Name: Protein disulfide-isomerase A3
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA3
• UniProt Synonym Gene Names: ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
• UniProt Entry Name: PDIA3_HUMAN

NCBI Description

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: Endoplasmic reticulum; EC 5.3.4.1; Phospholipase; Protease; Chaperone; Apoptosis; Isomerase

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: cell surface; smooth endoplasmic reticulum; focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus

Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; cell redox homeostasis; protein import into nucleus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; proteolysis; post-translational protein modification; signal transduction; protein retention in ER