Western Blot (WB)
(Mouse Trib3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Trib3 antibody detected the Trib3 protein (arrow).)
Rabbit anti-Mouse Trib3 Polyclonal Antibody | anti-Trib3 antibody
Trib3, CT (Trib3, Nipk, Trb3, Tribbles homolog 3, Neuronal cell death-inducible putative kinase) (APC)
Gene Names
Trib3; Nipk; SINK; Trb3; Ifld2; SKIP3; TRB-3
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Trib3; Polyclonal Antibody; CT (Trib3; Nipk; Trb3; Tribbles homolog 3; Neuronal cell death-inducible putative kinase) (APC); Neuronal cell death-inducible putative kinase; anti-Trib3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-Trib3 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Mouse Trib3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the C-terminal region of Mouse Trib3.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Trib3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Trib3 antibody detected the Trib3 protein (arrow).)
Western Blot (WB)
(Mouse Trib3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Trib3 antibody detected the Trib3 protein (arrow).)
Related Product Information for anti-Trib3 antibody
Trib3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Product Categories/Family for anti-Trib3 antibody
References
Morse, E., et al. Am. J. Physiol. Renal Physiol. 299 (5), F965-F972 (2010): Dedhia, P.H., et al. Blood 116(8):1321-1328(2010). Liew, C.W., et al. J. Clin. Invest. 120(8):2876-2888(2010). Humphrey, R.K., et al. J. Biol. Chem. 285(29):22426-22436(2010). Liu, J., et al. Am. J. Physiol. Endocrinol. Metab. 298 (3), E565-E576 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,023 Da
NCBI Official Full Name
tribbles homolog 3
NCBI Official Synonym Full Names
tribbles homolog 3 (Drosophila)
NCBI Official Symbol
Trib3
NCBI Official Synonym Symbols
Nipk; SINK; Trb3; Ifld2; SKIP3; TRB-3
NCBI Protein Information
tribbles homolog 3; induced in fatty liver dystrophy 2; neuronal cell death-inducible putative kinase
UniProt Protein Name
Tribbles homolog 3
Protein Family
UniProt Gene Name
Trib3
UniProt Synonym Gene Names
Nipk; Trb3; TRB-3
UniProt Entry Name
TRIB3_MOUSE
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Product Notes
The Trib3 trib3 (Catalog #AAA6358111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Trib3, CT (Trib3, Nipk, Trb3, Tribbles homolog 3, Neuronal cell death-inducible putative kinase) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Trib3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Trib3 trib3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trib3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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