Mouse RAD9A Monoclonal Antibody | anti-RAD9A antibody

RAD9A Monoclonal Antibody

Cat. Num. AAA9400203

• Gene Names: RAD9A; RAD9
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: RAD9A; Monoclonal Antibody; RAD9A Monoclonal Antibody; Cell cycle checkpoint control protein; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; anti-RAD9A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3A3-A7-F8
• Specificity: This antibody detects endogenous levels of RAD9A,and does not cross-react with related proteins.
• Purity/Purification: Affinity purified
• Form/Format: Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
• Sequence Length: 315

• Applicable Applications for anti-RAD9A antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western blotting: 1:500
• Immunogen Type: Recombinant Protein
• Immunogen Description: Purified recombinant human Rad9A protein fragments expressed in E Coli
• Target Name: RAD9A
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot detection of RAD9A in Hela,MCF7,3T3,COS7&Rat testis cell lysates using RAD9A antibody(1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody)

Related Product Information for anti-RAD9A antibody

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3'->5' double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Product Categories/Family for anti-RAD9A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 342672046
• NCBI GeneID: 5883
• NCBI Accession #: NP_001230153.1
• NCBI GenBank Nucleotide #: NM_001243224.1
• UniProt Accession #: Q99638; Q6FI29; Q96C41; B2RCZ8
• Molecular Weight: 42,547 Da
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 2
• NCBI Official Synonym Full Names: RAD9 checkpoint clamp component A
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: Cell cycle checkpoint control protein
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN

NCBI Description

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: EC 3.1.11.2; Apoptosis; Cell cycle regulation; Deoxyribonuclease; DNA replication

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; protein kinase binding; SH3 domain binding

Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA replication checkpoint; DNA repair; DNA catabolic process, exonucleolytic; DNA replication; response to DNA damage stimulus