Human Integrin Linked Kinase 1 ELISA Kit | ILK 1 elisa kit

Human Integrin Linked Kinase 1 ELISA Kit

Cat. Num. AAA727625

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human
• Synonyms: Integrin Linked Kinase 1; Human Integrin Linked Kinase 1 ELISA Kit; ILK 1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 25-500ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This ILK-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ILK-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ILK 1 elisa kit

Principle of the Assay: ILK-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ILK-1. Standards or samples are then added to the microtiter plate wells and ILK-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ILK-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ILK-1 are added to each well to "sandwich" the ILK-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ILK-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ILK-1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9408115
• NCBI GeneID: 3611
• UniProt Accession #: Q13418; P57043; Q68DZ3; B7Z418; D3DQU0
• Molecular Weight: 44,349 Da
• NCBI Official Full Name: integrin-linked kinase 1
• NCBI Official Synonym Full Names: integrin-linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase; integrin-linked kinase-2; epididymis secretory protein Li 28; 59 kDa serine/threonine-protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2
• UniProt Entry Name: ILK_HUMAN

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; MLK family; ILK subfamily

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: focal adhesion; costamere; protein complex; terminal button; intercellular junction; cytosol; nucleoplasm; sarcomere; membrane; cell soma; lamellipodium; cytoplasm; plasma membrane; stress fiber; dendritic shaft; cell junction

Molecular Function: integrin binding; protein serine/threonine kinase activity; signal transducer activity; protein binding; SH3 domain binding; protein kinase binding; ATP binding

Biological Process: negative regulation of smooth muscle cell proliferation; protein heterooligomerization; positive regulation of axon extension; positive regulation of transcription, DNA-dependent; cell-matrix adhesion; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; myelin formation; positive regulation of MAP kinase activity; positive regulation of cell proliferation; protein kinase B signaling cascade; fibril organization and biogenesis; negative regulation of neuron apoptosis; cell cycle arrest; positive regulation of cell-matrix adhesion; positive regulation of BMP signaling pathway; integrin-mediated signaling pathway; cell aging; positive regulation of dendrite morphogenesis; positive regulation of myoblast differentiation; myelination in the peripheral nervous system; establishment and/or maintenance of epithelial cell polarity; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cell proliferation; peptidyl-serine phosphorylation; ureteric bud branching; regulation of actin cytoskeleton organization and biogenesis; negative regulation of protein kinase activity; nerve development; positive regulation of phosphorylation; neurite morphogenesis; positive regulation of cell migration