Human Integrin-linked protein kinase ELISA Kit | ILK elisa kit

Human Integrin-linked protein kinase ELISA Kit

Cat. Num. AAA2883965

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human
• Synonyms: Integrin-linked protein kinase; Human Integrin-linked protein kinase ELISA Kit; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK; ILK; ILK1; ILK2; 2.7.11.1; ILK elisa kit

• Reactivity: Human
• Sequence Length: 452

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.401 ng/mL
• Intra-Assay CV: <=6.8%
• Inter-Assay CV: <=9.2%
• Recovery: 100%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ILK elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62420873
• NCBI GeneID: 3611
• NCBI Accession #: NP_001014794.1
• NCBI GenBank Nucleotide #: NM_001014794.2
• UniProt Accession #: Q13418; P57043; Q68DZ3; B7Z1I0; B7Z418; D3DQU0
• Molecular Weight: 36,467 Da
• NCBI Official Full Name: integrin-linked protein kinase isoform 1
• NCBI Official Synonym Full Names: integrin linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2
• UniProt Entry Name: ILK_HUMAN

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, TKL; TKL group; MLK family; ILK subfamily

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cell junction; cell soma; costamere; cytoplasm; cytosol; dendritic shaft; extracellular matrix; focal adhesion; intercellular junction; lamellipodium; membrane; nucleoplasm; plasma membrane; protein complex; sarcomere; stress fiber; terminal button

Molecular Function: ATP binding; integrin binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; SH3 domain binding; signal transducer activity

Biological Process: cell aging; cell cycle arrest; cell proliferation; cell-matrix adhesion; establishment and/or maintenance of epithelial cell polarity; fibril organization and biogenesis; integrin-mediated signaling pathway; myelin formation; myelination in the peripheral nervous system; negative regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; nerve development; neurite morphogenesis; peptidyl-serine phosphorylation; positive regulation of axon extension; positive regulation of BMP signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of dendrite morphogenesis; positive regulation of MAP kinase activity; positive regulation of myoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; protein kinase B signaling cascade; regulation of actin cytoskeleton organization and biogenesis; ureteric bud branching