Human Interleukin 4 Induced Protein 1 (IL4I1) ELISA Kit | IL4I1 elisa kit

Human Interleukin 4 Induced Protein 1 (IL4I1) ELISA Kit

Cat. Num. AAA454074

• Gene Names: IL4I1; LAO; FIG1; LAAO
• Reactivity: Human
• Synonyms: Interleukin 4 Induced Protein 1 (IL4I1); Human Interleukin 4 Induced Protein 1 (IL4I1) ELISA Kit; FIG1; LAAO; LAO; L-amino-acid oxidase; IL4I1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL4I1. No significant cross-reactivity or interference between IL4I1 and analogues was observed.
• Sequence Length: 589

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.052ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL4I1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL4I1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL4I1 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL4I1 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL4I1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL4I1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL4I1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL4I1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 384381475
• NCBI GeneID: 259307
• NCBI Accession #: NP_001244946.1
• NCBI GenBank Nucleotide #: NM_001258017.1
• UniProt Accession #: Q96RQ9; Q1WMJ3; Q4GZN1; Q4GZN2; Q6P2Q3; Q8TEM5; Q96RQ8
• Molecular Weight: 65,328 Da
• NCBI Official Full Name: L-amino-acid oxidase isoform 2
• NCBI Official Synonym Full Names: interleukin 4 induced 1
• NCBI Official Symbol: IL4I1
• NCBI Official Synonym Symbols: LAO; FIG1; LAAO
• NCBI Protein Information: L-amino-acid oxidase
• UniProt Protein Name: L-amino-acid oxidase
• UniProt Gene Name: IL4I1
• UniProt Synonym Gene Names: FIG1; LAAO; LAO; IL4-induced protein 1; hFIG1

NCBI Description

This gene encodes a protein with limited similarity to L-amino acid oxidase which contains the conserved amino acids thought to be involved in catalysis and binding of flavin adenine dinucleotide (FAD) cofactor. The expression of this gene can be induced by interleukin 4 in B cells, however, expression of transcripts containing the first two exons of the upstream gene is found in other cell types. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation ().